| 焦丽敏,梁进,王琼,等.卵巢癌组织中microRNA-7表达水平及其临床意义[J].安徽医药,2016,20(3):504-507. |
| 卵巢癌组织中microRNA-7表达水平及其临床意义 |
| Expression and clinical significance of microRNA-7 intissue of ovarian cancer patients |
| 投稿时间：2015-09-17 |
| DOI： |
| 中文关键词: 卵巢癌 微小RNA microRNA-7 RT-PCR |
| 英文关键词: ovarian carcinoma microRNA microRNA-7 RT-PCR |
| 基金项目:国家自然科学基金(No 81503526) |
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| 中文摘要: |
| 目的 探讨microRNA-7在卵巢癌组织中的表达水平及其临床意义。方法 收集该院确诊的卵巢癌患者42例,每例均行手术切除。同时收集38例良性卵巢上皮囊肿组织作为良性对照组。采用RT-PCR方法分别检测卵巢癌组织、癌旁组织和良性病变组织样本microRNA-7的表达,并分析其表达与卵巢癌临床特征的关系。结果 卵巢癌组织microRNA-7表达为0.176±0.063,癌旁组织表达为0.314±0.099,良性病变组织表达为0.465±0.123。与癌旁组织和良性病变组织比较,卵巢癌组织中microRNA-7 表达显著下降(P均＜0.05)。microRNA-7的表达与患者的年龄、病理分型无关,与临床分期和区域淋巴结转移相关(P均<0.05)。单因素分析结果显示,microRNA-7高表达的卵巢癌病人总生存期长于低表达组(P=0.02)。结论 microRNA-7在卵巢癌组织中表达下显著调,可能是卵巢癌患者重要的预后指标之一。 |
| 英文摘要: |
| Objective To explore the expression of microRNA-7 in tissue of ovarian cancer patients and to analyze the relationship between microRNA-7 and clinical features. Methods We enrolled 42 ovarian cancer patients and 38 controls with benign ovarian epithelial cyst in the study. By the reverse transcription-real time polymerase chain reaction (RT-qPCR), the expression of microRNA-7 was detected in tumor tissues, paracancerous tissues and benign tissues. Results Compared with the controls, microRNA-7 in the tissues of ovarian cancer patients was significantly reduced (P＜0.05). The expression of microRNA-7 in the tissues of ovarian cancer patients was not related to the age or pathological type, but to the clinical stage and metastasis (P<0.05). The patients with high microRNA-7 expression had better overall survival than the patients with low microRNA expression. Conclusion The expression of microRNA-7 in tissues of ovarian cancer patients is down regulated, which may become an important prognostic indicator for ovarian carcinoma. |
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