| 付曼妮,解翠林,石年.咪唑斯汀对小鼠接触性皮炎的治疗作用及机制研究[J].安徽医药,2016,20(5):836-838. |
| 咪唑斯汀对小鼠接触性皮炎的治疗作用及机制研究 |
| Therapeutic effect and discussion of mechanism of mizolastine on murine allergic contact dermatitisFU Man-ni,XIE Cui-lin,SHI Nian (Department of Dermatology,Huangshi Central Hospital Pu-Ai Branch,the Affiliated Hospital of Hubei Polytechnic University,Edong Healthcare Group,Huangshi 435000,China) Abstract:Objective |
| 投稿时间：2015-12-21 |
| DOI： |
| 中文关键词: 皮炎,接触性 肿瘤坏死因子α 白细胞介素4 白细胞介素12 小鼠 |
| 英文关键词: Dermatitis,Contact Dermatitis,Contact Interleukin-4 Interleukin-12 Mice |
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| 中文摘要: |
| 目的 研究咪唑斯汀对小鼠反应性接触性皮炎(ACD)的治疗作用,探讨其可能机制。 方法 采用2,4 – 二硝基氟苯(DNFB )建立小鼠背部ACD 模型,建模成功后将其随机分为模型组、咪唑斯汀组和阳性对照醋酸地塞米松组,每组10只,另外选取10只未处理小鼠作为对照组。用药组采取灌胃给药进行治疗,对照组和模型组给予同体积生理盐水。分别在用药后24 h和72 h测定各组皮肤厚度、皮肤肿胀度,ELISA法测定各组小鼠血清及皮肤组织TNF-α、IL-4、 IL-12的水平。 结果 与对照组相比,ACD组皮肤厚度和皮肤肿胀度显著增加 (P＜0.05 )；血清及皮损组织中TNF-α、IL-12水平显著升高( P＜0.05 ),而IL-4水平无明显变化(P＞0.05)。咪唑斯汀能显著抑制ACD小鼠皮肤肿胀及TNF-α、IL-12 水平,对IL-4水平无明显影响。 结论 咪唑斯汀对ACD模型小鼠有良好的治疗作用,可能与降低Th1型炎症因子水平有关。 |
| 英文摘要: |
| Objective To study the efficacy of Mizolastine in the treatment of allergic contact dermatitisin (ACD) mice and its possible mechanisms. Methods The DNFB was applied on the dorsum of mice to produce the allergi-c contact dermatitis models.Total of forty mice were randomly divided in to nor mal control group,model group,mizolastine group,dexamethasone acetate group with 10 mice in each group.At 24 hours and 72 hours after the beginning of treatmen,central thickness and the swelling of dorsal skins were examined.The leve ls of TNF-α,IL-12,IL-4 in serum and tissue of skin lesion were determined by ELISA method. Results Compared with the controlgroup,the ACD group showed a significant increase in thickness and swelling degree of dorsal skin,levels of T-NF-α,IL-12 in serum and tissue of skin lesion (P＜0.05).The levels of IL-4 in serum and tissue of skin lesion were not changed (P＞0.05).Application of the mizolastine can effectively suppress swelling degree of dorsal skin,reduce levels of TNF-α,IL-12,but did not cause significant changes in serum levels of IL-4 in mice with ACD. Conclusion Mizolastine had good th erapeutic effect on allergic contact dermatitis mode1 of mice,and the mechanism might be related to suppression of Th1 cytokine. |
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