| 史艳玲,李振华,朱亚楠,等.高效液相色谱法测定复方虎杖颗粒中盐酸小檗碱含量[J].安徽医药,2016,20(6):1072-1074. |
| 高效液相色谱法测定复方虎杖颗粒中盐酸小檗碱含量 |
| Determination of berberine hydrochloride in compound polygoni cuspidati rhizoma et radix grain by HPLC |
| 投稿时间：2016-01-07 |
| DOI： |
| 中文关键词: 虎杖 小檗碱 复方 色谱法,高压液相 |
| 英文关键词: Polygonum cuspidatum Berberine Compounds(tcd) Chromatography,high pressure liquid |
| 基金项目:江苏省“六大人才高峰” 高层次人才培养对象科研资助项目(2015-YY-011)；江苏省“333高层次人才培养工程”科研资助项目(BRA2015494) |
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| 中文摘要: |
| 目的 建立复方虎杖颗粒中盐酸小檗碱的含量测定方法。方法 采用高效液相色谱法(HPLC),色谱柱为Alltima C18 柱(4.6 mm×250 mm,5 μm),流动相为乙腈-0.1%磷酸-十二烷基磺酸钠 (50∶50∶0.1),流速为1 mL·min-1；检测波长为265 nm。结果 盐酸小檗碱在0.046～0.460 μg范围呈良好的线性关系(r=0.999 9),平均回收率为100.79%,RSD为2.6%(n=6)。结论 该方法简便、快速、重现性好,可以更好地对复方虎杖颗粒的质量进行控制。 |
| 英文摘要: |
| Objective To establish a method for determination of berberine hydrochloride in compound polygoni cuspidati rhizoma et radix grain by HPLC.Methods The analysis was performed on an Alltima-C18 column using acetonitrile-0.1% phosphoric acid (50∶50) (0.1 g sodium dodecyl sulfonate was added into 100 mL solution) as the mobile phase.The flow rate was 1 mL·min-1 and the detection wavelength was set at 265 nm.Results Berberine hydrochloride showed a good linear relationship within the range of 0.046～0.460 μg (r=0.999 9),and the average recovery of berberine hydrochloride was 100.79%,RSD=2.6%.Conclusions The method is rapid,simple and accurate,and it can be used in the quality control of compound polygoni cuspidati rhizoma et radix. |
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