| 谢议凤,王站旗,苏佩玲.白花丹素抑制肾小球系膜细胞增殖及促纤维化相关因子的表达[J].安徽医药,2016,20(8):1446-1449. |
| 白花丹素抑制肾小球系膜细胞增殖及促纤维化相关因子的表达 |
| Inhibition effect of plumbagin on proliferation of human glomerular mesangial cell and the mechanisms |
| 投稿时间：2016-05-18 |
| DOI： |
| 中文关键词: 白花丹 转化生长因子β1 结缔组织生长因子 连接蛋白类 |
| 英文关键词: Plumbago zeylanica Transforming growth factor beta1 Connective tissue growth factor Connexins |
| 基金项目: |
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| 中文摘要: |
| 目的 探讨白花丹素对人肾小球系膜细胞株HMC细胞增殖及转化生长因子-β1(TGF-β1)、结缔组织生长因子(CTGF)、纤维连接蛋白(FN)表达的影响。 方法 1~10 μmol·L-1白花丹素分别处理HMC细胞后,MTT法检测细胞增殖活性；RT-PCR和细胞免疫荧光法分别检测TGF-β1、CTGF、FN mRNA和蛋白的表达。结果 1~10 μmol·L-1白花丹素作用后,HMC细胞呈时间、浓度依赖性生长抑制,TGF-β1、CTGF、FN mRNA和蛋白的表达下降。结论 白花丹素通过降低HMC细胞TGF-β1、CTGF、FN的表达,抑制HMC细胞增殖。 |
| 英文摘要: |
| Objective To investigate the effects of plumbagin (PL) on the proliferation of human mesangial cell (HMC) cultured in vitro and the expression of TGF-β1,CTGF,FN in mRNA and protein level.Methods The experiment subjects were randomized into control group and five PL groups with different concentrations (1.0,2.5,5.0,7.5,0.0 μmol·L-1),which were treated with DMSO and PL respectively.The proliferation activity of mesangial cells was observed by MTT assay after 24,8,72 hours.The expressions of TGF-β1,CTGF,FN mRNA and protein were detected by RT-PCR and immunofluorescence after 24 hours.Results Absorbance recorded at 490 nm wavelength at different time in PL group was lower than that of control group(P<0.05).Electrophoresis strip density of PCR product and absorbance of immunofluorescence in PL group was significantly lower than that of control group(P<0.05),and a dose and time response relationship was shown in results.Conclusions Plumbagin can inhibit the proliferation of glomerular mesangial cell by downregulating the expressions of TGF-β1,CTGF,FN in mRNA and protein level. |
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