| 赵荣,李闻涛,董彬厂,等.药用沙棘籽油质量标准研究[J].安徽医药,2016,20(8):1479-1482. |
| 药用沙棘籽油质量标准研究 |
| Quality standard for seeds oil of Hippophae rhamnaides L. |
| 投稿时间：2016-04-21 |
| DOI： |
| 中文关键词: 沙棘/标准 色谱法,薄层 维生素E |
| 英文关键词: Hippophae rhamnoides/standards Chromatography,thin layer Vitamin E |
| 基金项目: |
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| 摘要点击次数: 2334 |
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| 中文摘要: |
| 目的 研究药用沙棘籽油的质量标准控制方法。方法(1)采用系列薄层色谱法(TLC)对药用沙棘籽油进行鉴别。(2)采用HPLC法测定药用沙棘籽油中的维生素E。色谱柱:Kromasil-C18(250 mm× 4.6 mm,5 μm)；流动相:甲醇-乙腈(78∶22)；柱温:30 ℃；检测波长:280 nm；流速:1.0 mL·min-1 。结果(1)采用系列TLC 法对药用沙棘籽油进行鉴别具有较好的专属性。(2)维生素E在0.197 2～1.972 mg·L-1的范围内线性关系良好(r=0.999 7),平均回收率为99.6%(RSD=0.92%,n=6)。结论 所建立的标准科学合理,可用于药用沙棘籽油的质量控制。 |
| 英文摘要: |
| Objective To establish the quality standard for Seeds Oil of Hippophae rhamnaides L.Methods The Seeds Oil of Hippophae rhamnaides L.was identified by a series of TLC methods.HPLC was adopted to determine the content of Vitamin Ein the seeds oil of Hippophae rhamnaides L.Kromasil-C18 column(250 mm× 4.6 mm,5 μm) was used with methanol-acetonitrile(78∶22) as the mobile phase.The column temperature was set at 30 ℃ with a detection wavelength of 280 nm and a flow rate at 1.0 mL·min-1.Results A series of TLC methods for the identification of the seeds oil of Hippophae rhamnaides L.showed good specificity.For Vitamin E,there was a good linear relationship within the range of 0.197 2～1.972 mg·L-1(r=0.999 5).The average recovery was 99.6% (RSD=0.92%,n=6).Conclusions The standard can be use for the quality control of Seeds Oil of Hippophae rhamnaides L. |
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