| 任玲,张光哲,邢承忠.芬太尼抑制鼻咽癌细胞侵袭转移的机制研究[J].安徽医药,2016,20(8):1593-1596. |
| 芬太尼抑制鼻咽癌细胞侵袭转移的机制研究 |
| Mechanism of fentanyl inhibiting invasion and metastasis of nasopharyngeal carcinoma cells |
| 投稿时间：2016-05-16 |
| DOI： |
| 中文关键词: 鼻咽肿瘤 芬太尼 src族激酶类 肿瘤侵润 肿瘤转移 药理作用分子作用机制 |
| 英文关键词: Nasopharyngeal neoplasms Fentanyl Src-family kinases Neoplasm invasiveness Neoplasm metastasis Molecular mechanisms of pharmacological action |
| 基金项目: |
|
| 摘要点击次数: 2720 |
| 全文下载次数: 183 |
| 中文摘要: |
| 目的 通过选择10 nmol·L-1芬太尼作用于鼻咽癌细胞系CNE2和HONE1,研究芬太尼对鼻咽癌细胞迁移能力的影响。方法 取对数生长期的鼻咽癌细胞系CNE2和HONE1,选择10 nmol·L-1芬太尼分别作用于鼻咽癌细胞系30 min和1 h,Western blot检测p-Src和Src表达,Transwell检测细胞迁移能力；构建Src过表达质粒,转染鼻咽癌细胞系,观察鼻咽癌细胞形态,检测细胞的迁移能力。结果 10 nmol·L-1芬太尼处理鼻咽癌细胞CNE2和HONE1 48 h后,细胞呈现出类间质向上皮转化的形态特征,同时,与对照组相比,加入芬太尼处理组的鼻咽癌细胞其迁移能力显著降低；应用芬太尼分别处理鼻咽癌细胞30 min和1 h后,p-Src水平显著下调；此外,与转染空载体质粒的对照组相比,过表达Src的细胞迁移能力明显增强,而在过表达Src的细胞中同时联合芬太尼处理后,细胞的迁移能力没有被抑制。结论 芬太尼可能通过抑制Src通路活化抑制鼻咽癌细胞侵袭转移。 |
| 英文摘要: |
| Objective To investigate the effect of fentanyl on nasopharyngeal carcinoma cells(NPC) metastasis by treating NPC lines CNE2 and HONE1 with fentanyl (10 nmol·L-1) in this study.Methods Nasopharyngeal carcinoma cells (CNE2 and HONE1) in logarithmic growth phase were pretreated with fentanyl (10 nmol·L-1) for 30 min and 1 hour.Western blot was used to test the expression levels of p-Src and Src and Transwell test was used to evaluate cell metastatoc ability.Src over-expression plasmid was constructed and transfected into NPC cell lines and NPC cellular morphology and metastatic ability were observed.Results NPC cell lines (CNE2 and HONE1) exhibited mesenchymal-to-epithelial like transformation;furthermore,the metastatic ability of NPC cells decreased obviously compared with control group 48 hours after fentanyl treatment.The p-Src expression level was down-regulated after fentanyl treatment for 30 min and 1 hour.However,over-expressive Src enhanced the metastatic ability of NPC cells obviously,and in combination with fentanyl treatment over-expressive Src plasmid transfection did not inhibit the metastatic ability of NPC cells.Conclusions Fentanyl may inhibit nasopharyngeal carcinoma cells by inhibiting Src pathway invasion and metastasis. |
|
查看全文
查看/发表评论 下载PDF阅读器 |
| 关闭 |
|
|
|
