| 曾俊芬,王新桂,鲁建武,等.桂枝汤方剂不同提取部位免疫抑制活性的考察[J].安徽医药,2016,20(9):1629-1632. |
| 桂枝汤方剂不同提取部位免疫抑制活性的考察 |
| Screening for immunosuppressive function on different fraction of Guizhi decoction |
| 投稿时间：2016-03-23 |
| DOI： |
| 中文关键词: 桂枝汤/分析 化学分馏法 超敏反应,迟发型 催化域 |
| 英文关键词: |
| 基金项目: |
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| 摘要点击次数: 2894 |
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| 中文摘要: |
| 目的 筛选桂枝汤方剂免疫抑制活性部位。方法 处方药材分别水煎浓缩为水提部位,乙醇提取为乙醇提取部位。同时将乙醇部位分别用石油醚、氯仿、乙酸乙酯、正丁醇萃取得石油醚部位、氯仿部位、乙酸乙酯部和正丁醇部位。CCK-8法考察桂枝汤不同提取物对ConA激活T细胞和LPS激活B细胞增殖的抑制作用。同时用二硝基氯苯对昆明种小鼠皮肤致敏和诱发,制成迟发型超敏反应模型,观察桂枝汤方剂不同部位对迟发型超敏反应的干预作用。结果 桂枝汤方剂水提物、乙醇提取物、石油醚萃取部位有显著免疫抑制功效,乙醇和石油醚提取物效果最佳。浓度200 mg·L-1时T细胞增殖抑制率分别为48.3%、45.9%,B细胞抑制率大于30%。乙醇提取物、石油醚萃取部位对迟发型超敏反应小鼠耳廓肿胀有显著抑制作用,对胸腺和脾脏无明显影响。结论 桂枝汤方剂乙醇提取物和石油醚萃取物为免疫抑制活性部位,能显著抑制T细胞和B细胞的增殖,抑制小鼠耳廓肿胀,对正常细胞、胸腺、脾脏无毒性。 |
| 英文摘要: |
| Objective To screen the immunosuppressive fraction of Guizhi decoction.Methods Medicinal materials of Guizhi decoction was extracted by water and ethanol to get water extraction and ethanol extraction.Ethanol extraction was extracted with petroleum ether,chloroform,ethyl acetate,n-butanol to get petroleum ether fraction,chloroform fraction,ethyl acetate fraction,and n-butanol fraction respectively.CCK-8 method was used to detect the activity of different extracts on lymphocytes in the spleen of mice.ConA and LPS were added to the spleen cells of BALB/c mice to activate Tcells and Bcells respectively.Different extracts of Guizhi decoction were added to the activated spleen cells to investigate the immunosuppressive effect.The model of delayed-type hypersensitivity was established by sensitizing the skin of Kunming mice with DNCB.Effects of different fractions of Guizhi decoction were observed on delayed-type hypersensitivity mice.Results Water extraction,ethanol extraction and petroleum ether fraction had significant immunosuppression.Ethanol extraction and petroleum ether fraction showed the greatest activities with inhibitory rates of Tcells 48.3% and 45.9%,and Bcells inhibitory rate was more than 30% at the concentration of 200 mg·L-1.Ethanol extraction and petroleum ether fraction could alleviate obviously the degree of swollen-auricle of mice and without significant influence on spleen and thymus.Conclusions The ethanol extraction and petroleum ether fraction were the main immunosuppressive fraction,which could significantly inhibit the proliferation of Tand Bcells and alleviate the swollen-auricle of mice without toxicity to normal cells and spleen and thymus. |
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