| 王之学,肖继州,赵振霞,等.纳米微囊介导的碱性成纤维细胞生长因子联合低氧诱导因子-1提高随意型皮瓣缺氧耐受的实验研究[J].安徽医药,2016,20(9):1645-1648. |
| 纳米微囊介导的碱性成纤维细胞生长因子联合低氧诱导因子-1提高随意型皮瓣缺氧耐受的实验研究 |
| An experimental study on nanoparticle mediatedapplication with bFGF and HIF-1 to increase hypoxia toleranceof random skin flap |
| 投稿时间：2016-03-18 |
| DOI： |
| 中文关键词: 外科皮瓣 纳米囊 成纤维细胞生长因子 芳香烃受体核转位子 新生血管化,生理性 大鼠 |
| 英文关键词: |
| 基金项目: |
|
| 摘要点击次数: 2617 |
| 全文下载次数: 78 |
| 中文摘要: |
| 目的 探讨以纳米微囊为载介导的碱性成纤维细胞生长因子(bFGF)联合低氧诱导因子-1(HIF-1)对大鼠随意性皮瓣缺氧耐受的作用。方法 SD大鼠40只,背部设计6 cm×2 cm的随意性皮瓣。采用随机方法分为4组:A组为联合应用含bFGF和HIF-1的观察组；B组为单纯应用bFGF的基因对照组1；C组为单纯应用HIF-1的基因对照组2；D组为应用生理盐水的空白对照组。采用皮瓣下注射的方式,术后测量皮瓣成活面积,病理切片HE染色,皮瓣组织的bFGF和HIF-1免疫组化检测及皮瓣下毛细血管的数目密度统计。结果 纳米微囊介导的观察组与其他对照组相比,皮瓣成活率更高,目的蛋白表达和皮瓣新生血管密度计数与对照组差异有统计学意义(P<0.05)。结论 以纳米微囊为载体,联合应用bFGF和HIF-1的基因治疗能促进皮瓣血管的增生,显著提高大鼠随意性皮瓣的缺氧耐受,增加其成活率。 |
| 英文摘要: |
| Objective To investigate the impact of basic fibroblast growth factor(bFGF) and hypoxia-induced factor -1(HIF-1) mediated by nanoparticleon hypoxia tolerance of random skinflap in rats.Methods We designedone random skin flap of 6cm×2cm on the back of SD rat(n=40).Then rats were randomized into four groups:group Aas the experimental group with application of bFGF and HIF-1;group Bas the first control group with soleapplication ofbFGF;group Cas the second control group with sole application of HIF-1 and group Das blank control group with application of normal saline only.Results The exprimental group which was mediated bynanoparticle,compared with the other control groups,achieved higher survival rate of skin flap.Conclusions Gene therapy with bFGF and HIF-1 mediated by nanoparticle can promote the neovascularization in skinflap,enhance the hypoxia tolerance and increase the survival rate of random skin flap of rats. |
|
查看全文
查看/发表评论 下载PDF阅读器 |
| 关闭 |
|
|
|
