| 袁杰,张新艳.高效液相色谱梯度洗脱法同时测定冠心苏合滴丸中5个成分的含量[J].安徽医药,2016,20(9):1651-1654. |
| 高效液相色谱梯度洗脱法同时测定冠心苏合滴丸中5个成分的含量 |
| Simultaneous determination of contents of five components in Guanxin Suhe Dripping Pills by HPLC with gradient elution method |
| 投稿时间：2016-04-11 |
| DOI： |
| 中文关键词: 中草药/分析 滴丸剂 心绞痛 色谱法,高压液相 |
| 英文关键词: |
| 基金项目: |
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| 摘要点击次数: 3063 |
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| 中文摘要: |
| 目的 建立高效液相色谱(HPLC)梯度洗脱法同时测定冠心苏合滴丸中的马兜铃酸Ⅰ、荭草苷、异荭草苷、牡荆苷和异牡荆苷含量。方法 采用Diamonsil C18色谱柱(250 mm×4.6 mm,5 μm),以甲醇(A)-0.5％冰醋酸溶液(B)为流动相,进行梯度洗脱,流速0.9 mL·min-1,柱温30 ℃,波长切换法检测(0～21 min,390 nm,马兜铃酸Ⅰ；21～60 min,338 nm,荭草苷、异荭草苷、牡荆苷和异牡荆苷)；进样量为10 μL。结果 马兜铃酸Ⅰ、荭草苷、异荭草苷、牡荆苷和异牡荆苷5个成分的线性范围分别为4.16～83.20 mg·L-1(r=0.999 6)、6.35～127.00 mg·L-1(r=0.999 8)、4.98～99.60 mg·L-1(r=0.999 2)、14.33～286.60 mg·L-1(r=0.999 7)、16.37～327.40 mg·L-1(r=0.999 4)；平均加样回收率及相应的RSD分别为98.26%(1.40%),99.23%(1.35%),97.90%(1.58%),96.97%(1.14%),99.11%(1.58%)。结论 该文建立的方法符合方法学验证要求,可用于冠心苏合滴丸中的5个指标性成分的同时定量测定。 |
| 英文摘要: |
| Objective To develop an HPLC with gradient elution method for determination of the contents of aristolochic acid Iorientin,isoorientin,vitexin and isovitexin in Guanxin Suhe Dripping Pills simultaneously.Methods The Diamonsil C18 (250 mm×4.6 mm,5 μm) chromatographic column was adopted;the mobile phase was methanol(A)-0.5% glacial acetic acid solution(B) with gradient elution at a flow rate of 0.9 mL·min-1.Sample quantity was 10 μL,the column temperature was set at 30 ℃,and the detection wavelengths were 390 nm(0~21 min,aristolochic acid I),338 nm(21~60 min,orientin,isoorientin,vitexin and isovitexin).Results Good linear relationships of aristolochic acid I,orientin,isoorientin,vitexin and isovitexin,were found in the ranges of 4.16~83.20 mg·L-1 (r=0.999 6),6.35~127.00 mg·L-1 (r=0.999 8),4.98~99.60 mg·L-1 (r=0.999 2),14.33~286.60 mg·L-1 (r=0.999 7),and 16.37~327.40 mg·L-1 (r=0.999 4),respectively.The average recoveries and the corresponding RSD were 98.26%(1.40%),99.23%(1.35%),97.90%(1.58%),96.97%(1.14%),and 99.11%(1.58%),respectively.Conclusions This method is consistent with the method validation requirements,and can simultaneously determine the above 5 index components of Guanxin Suhe Dripping Pills. |
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