| 朱明岩,叶英.不同荧光定量PCR技术在乙型肝炎病毒检测中的应用评价[J].安徽医药,2016,20(9):1723-1726. |
| 不同荧光定量PCR技术在乙型肝炎病毒检测中的应用评价 |
| Application evaluationof different PCR technologies in detecting HBV-DNA |
| 投稿时间：2016-03-04 |
| DOI： |
| 中文关键词: 乙型肝炎病毒 多重聚合酶链式反应 荧光抗体技术 |
| 英文关键词: |
| 基金项目:国家自然科学基金项目(81172737) 基因(Hepatitis B Virus gene,HBV DNA)载量在评价治疗指征、治疗效果及耐药的发生方面具有非常重要的价值。罗氏内标法Cobas检测是国际上公认的标准,然而试剂价格昂贵,在我国难以广泛使用。目前我国已批准实时荧光定量PCR试剂用于HBV DNA检测,但与罗氏Cobas检测在结果上的差异尚 |
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| 中文摘要: |
| 目的 评价普通荧光定量PCR技术与罗氏内标法荧光定量PCR技术在乙型肝炎病毒(HBV)检测中的意义。方法 采集HBV患者的血清190份,分别用两种方法进行检测,通过相关性分析和Kappa检验评价两种试剂检测结果之间的差异性及一致性。结果(1)190份标本中,罗氏Cobas试剂与国产试剂检测结果呈线性相关(R2=0.670 7,P<0.05)；(2)分成HBeAg(+)与HBeAg(-)两组比较,两组中Cobas试剂与国产试剂检测均呈线性相关(R2=0.586 4,P<0.05；R2 =0.522 6,P<0.05)；(3)根据不同HBeAg状态和抗病毒要求的DNA载量对标本进行一致性分析,HBeAg(+)组Kappa值为0.752；HBeAg(-)组Kappa值为0.381。结论 对于HBeAg(+)患者,可以应用国产试剂检测患者的HBV-DNA水平,而对于HBeAg(-)患者,建议应用Cobas试剂检测其患者的HBV-DNA水平。 |
| 英文摘要: |
| Objective To evaluate the application of domestic PCR technology and Cobas Ampli Prep/CobasTaqMan 48 PCR technology in detecting HBV-DNA.Methods Wecollected 190 serum samples from patients with chronic HBV infections in The First Affiliated Hospital of Anhui Medical University and the samples were monitored by domestic reagent and CobasTaqMan reagent.The performances of the two reagents were compared and analyzed by correlation analysis and Kappa test.Results The 190 serum samples were tested by two assays and there wassignificant linear correlation betweenthe two methods(R2=0.670 7,P<0.05).The serum samples were assigned into HBeAg-positive and HBeAg-negative groups.The data of two test methods had significantlinear correlation(R2=0.586 4,P<0.05；R2=0.522 6,P<0.05).The conformity analysis showed that the Kappa values were 0.752 in HBeAg-postive group and 0.381 in HBeAg-negative group.Conclusions The results of two test methods had poor conformity.CobasTaqMan reagent can be used to monitor HBV-DNA quantities in HBeAg-negative patients while domestic reagent canbe used in HBeAg-positive group. |
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