| 王玉林,夏方亮.高效液相色谱法测定天杞补肾胶囊中马钱苷、补骨脂素和异补骨脂素的含量[J].安徽医药,2016,20(10):1865-1867. |
| 高效液相色谱法测定天杞补肾胶囊中马钱苷、补骨脂素和异补骨脂素的含量 |
| Determination of loganin,psoralen and isopsoralenin tianqibushen capsules by HPLC |
| 投稿时间：2016-05-06 |
| DOI： |
| 中文关键词: 天杞补肾胶囊 马钱苷 补骨脂素 异补骨脂素 |
| 英文关键词: Tianqibushen capsule Loganin Psoralen Isopsoralen |
| 基金项目: |
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| 中文摘要: |
| 目的 建立高效液相色谱法(HPLC)测定天杞补肾胶囊中马钱苷、补骨脂素和异补骨脂素的含量测定方法。 方法 用 C18 柱为固定相,以甲醇-水(42∶58)为流动相,检测波长为240 nm。 结果 马钱苷、补骨脂素和异补骨脂素分别在5.06～126.5、0.527 5～13.187 0、0.480 8～12.020 0 mg·L-1范围内呈良好的线性关系,R2分别为1.000、1.000、1.000(n＝6),其平均回收率分别为98.93%,100.09%和98.97%。 结论 该方法具有较高的准确性和专属性。 |
| 英文摘要: |
| Objective To establish a method for the determination of Loganin,Psoralen and Isopsoralen in Tianqibushen Capsules by HPLC.Methods The C18 column was used,the mobile phase was methanol-water(42∶58),and the detection wavelength was 240 nm. Results The standard curves of Loganin,Psoralen and Isopsoralen were linear in the ranges of 5.06～126.5 mg·L-1(R2=1.000,n=6),0.527 5～13.187 0 mg·L-1(R2=1.000,n=6) and 0.480 8～12.020 0 mg·L-1(R2=1.000,n=6) respectively,and the average recoveries were 98.93%,100.09% and 98.97%. Conclusion The method is highly accurate and specific. |
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