| 刘仔,蓬展鹏,邝惠珍,等.金沙牛化石片高效液相色谱指纹图谱研究[J].安徽医药,2016,20(11):2043-2045. |
| 金沙牛化石片高效液相色谱指纹图谱研究 |
| HPLC fingerprint of jinshaniu huashi tablets |
| 投稿时间：2016-06-24 |
| DOI： |
| 中文关键词: 金沙牛化石片 高效液相色谱 指纹图谱 |
| 英文关键词: Jinshaniu huashi tablets HPLC Fingerprint金项目:广东省东莞市科技计划项目(2003) |
| 基金项目:广东省东莞市科技计划项目(2003) |
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| 摘要点击次数: 3228 |
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| 中文摘要: |
| 目的 建立金沙牛化石片高效液相色谱(HPLC)法指纹图谱,为科学评价金沙牛化石片质量及其生产工艺稳定性提供依据。方法 采用AgiLent ZORBAX SB-C18色谱柱(4.6 mm×150 mm,5 μm),以0.2%磷酸-乙腈为流动相进行梯度洗脱,流速为1.0 mL·min-1,检测波长为254 nm,柱温为25 ℃。采用国家药典委员会“中药色谱指纹图谱相似度评价系统(2012版)”对10批金沙牛化石片进行相似度评价。结果 以夏佛塔苷为参照峰,初步建立了金沙牛化石片HPLC指纹图谱,确定了18个共有峰,10批金沙牛化石片样品指纹图谱相似度均在0.9以上。结论 该方法简便、准确、重复性好,可用于金沙牛化石片质量控制。 |
| 英文摘要: |
| Objective To establish HPLC fingerprints of jinshaniu huashi tablets,to provide basis for the scientific assessments of jinshaniu huashi tablets’ quality and stability of the production process.Methods The separation was performed on a ZORBAX SB-C18 column(4.6 mm×150 mm,5 μm)with 0.2% phosphoric acid-acetonitrile as the mobile phase in a gradient elution at a flow rate of 1.0 mL·min-1.The detection wavelength was set at 254 nm,and the column temperature was set at 25 ℃.Fingerprint Similarity Evaluation Software(edition 2012)of Chinese Pharmacopoeia Commission was used to evaluate the similarity of the 10 batches of Jinshaniu Huashi Tablets.Results The common mode for the HPLC fingerprint was set up with schaftoside as the reference peak.Eighteen co-processing peaks were selected as the fingerprint peaks of jinshaniu huashi tablets.Good similarities with correlation coefficients higher than 0.9 were found between 10 batches of jinshaniu huashi tablets and the standard fingerpint.Conclusions The method is simple,accurate,and reproducible,which could be used for quality control of jinshaniu huashi tablets. |
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