| 张婷.高效液相色谱法同时测定小儿咳喘灵口服液中绿原酸与甘草苷的含量[J].安徽医药,2016,20(11):2052-2054. |
| 高效液相色谱法同时测定小儿咳喘灵口服液中绿原酸与甘草苷的含量 |
| Determination of chlorogenic acid and liquiritin in xiao’er kechuanling oral liquid by HPLC |
| 投稿时间：2016-06-28 |
| DOI： |
| 中文关键词: 小儿咳喘灵口服液 高效液相色谱法 绿原酸 甘草苷 |
| 英文关键词: Xiao’er kechuanling oral liquid HPLC Chlorogenic acid Liquiritin |
| 基金项目: |
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| 摘要点击次数: 3496 |
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| 中文摘要: |
| 目的 建立采用高效液相色谱法(HPLC)同时测定小儿咳喘灵口服液中绿原酸与甘草苷含量的方法。方法 采用Agilent Eclipse Plus C18(150 mm×4.6 mm,5 μm)色谱柱；流动相为乙腈-0.1%磷酸溶液,梯度洗脱,流速为1.0 mL·min-1；波长切换检测,检测波长为327 nm(0～16 min,测定绿原酸)和217 nm(16～64 min,测定甘草苷)；进样量为10 μL；柱温为30℃。结果 绿原酸在0.081 3～0.812 8 μg范围内呈良好的线性关系(r=0.999 8),平均加样回收率为97.5%(n=9),RSD=2.8%；甘草苷在0.038 6～0.386 1 μg范围内呈良好的线性关系(r=0.999 8),平均加样回收率为103.6%(n=9),RSD=1.9%。结论 该方法操作简便、灵敏度高、重现性好,适用于小儿咳喘灵口服液中绿原酸与甘草苷的含量测定。 |
| 英文摘要: |
| Objective To develop an HPLC method for the determination of chlorogenic acid and liquiritin in xiao’er kechuanling oral liquid.Methods The samples were analyzed by Agilent Eclipse Plus C18(150 mm×4.6 mm,5 μm)with acetonitrile-0.1% phosphoric acid as mobile phase in gradient elution at the flow rate of 1.0 mL·min-1.The detection wavelength was set at 327 nm for the determination of chlorogenic acid(0～16 min)and 217nm for the determination of liquiritin(16～64 min).The injection volume was 10 μL and the column temperature was 30 ℃.Results The linear range of chlorogenic acid was within 0.081 3～0.812 8 μg(r=0.999 8)with an average recovery of 97.5%(n=9,RSD=2.8%).The linear range of liquiritin was within 0.038 6～0.386 1 μg(r=0.999 8)with an average recovery of 103.6%(n=9,RSD=1.9%).Conclusion The method is convenient,highly sensitive and reproducible for the quantitative analysis of xiao’er kechuanling oral liquid. |
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