| 冒群,练维,徐爱宁.美罗培南脂质体冻干粉的制备及对耐药沙门菌抑制作用的研究[J].安徽医药,2016,20(12):2244-2247. |
| 美罗培南脂质体冻干粉的制备及对耐药沙门菌抑制作用的研究 |
| Preparation ofmeropenem liposome-lyophilized powder and its inhibitory effect on drug resistant salmonella |
| 投稿时间：2016-06-13 |
| DOI： |
| 中文关键词: 美罗培南 脂质体 体外释放 沙门菌 |
| 英文关键词: Meropenem Liposome In vitro release Salmonella |
| 基金项目: |
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| 摘要点击次数: 3456 |
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| 中文摘要: |
| 目的 研制一种长效、安全的新型美罗培南脂质体冻干粉,解决突发战争或紧急灾害条件下创伤感染缺乏长效抑菌药物的现状。方法 用薄膜分散法制备美罗培南脂质体,筛选葡萄糖、乳糖和甘露醇3种保护剂,经冷冻干燥制成冻干剂。透射电镜观察脂质体形态并摄制照片,对其含量、稳定性和体外释药性进行检测,并采用模拟药敏纸片法对其抑菌效果进行评估。结果 美罗培南脂质体外观呈球形,分布均匀,平均粒径为(98.1±8.2) nm,多分散系数(PDI)为0.208,包封率为(76.51±2.33)%,5%甘露醇为冻干保护剂最好,体外释药模式显示药物有缓释的特征,抑菌实验显示美罗培南脂质体具有明显的长效抑菌作用。结论 薄膜分散法制备美罗培南脂质体具有可行性,脂质体性质稳定,粒径较小,具有缓释效果,抑菌效果好。 |
| 英文摘要: |
| Objective To develop a new effective and safe body of meropenem liposome lyophilized powder in view of the lack of long-acting antibacterial drugs under field combat conditions.Methods Meropenem liposome was prepared by film dispersion method,and glucose,lactose and mannitol were selected as protective agents,and they were freeze-dried into lyoprotectants.The content of meropenem liposome was determined by ultraviolet spectrophotometry,and disk diffusion technique (K-B method) was used to evaluate the antibacterial effect of meropenem liposome.Results The average particle diameter of meropenem liposome was (98.1±8.2) nm,the polydispersity index (PDI) was 0.208,the entrapment efficiency was (76.51±2.33)%,and the 5% mannitol was the best dry protective agent.In vitro release test results showed that it had a significant sustained-release effect,and it was beneficial to long-acting bacteriostasis.Conclusions The process of meropenem liposome by film dispersion method is simple and feasible,and it had a high EE,smaller particle size,good stability and antibacterial effect. |
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