高效液相色谱法测定云南红药胶囊中的三七皂苷R<sub>1</sub>、重楼皂苷Ⅶ、重楼皂苷Ⅵ、重楼皂苷Ⅱ和重楼皂苷Ⅰ的含量

张铭; 袁杰; 张新艳

文章摘要

张铭,袁杰,张新艳.高效液相色谱法测定云南红药胶囊中的三七皂苷R₁、重楼皂苷Ⅶ、重楼皂苷Ⅵ、重楼皂苷Ⅱ和重楼皂苷Ⅰ的含量[J].安徽医药,2016,20(12):2257-2261.

高效液相色谱法测定云南红药胶囊中的三七皂苷R₁、重楼皂苷Ⅶ、重楼皂苷Ⅵ、重楼皂苷Ⅱ和重楼皂苷Ⅰ的含量

HPLC method for simultaneous determination of notoginsenoside R₁,chonglousaponin Ⅶ,chonglousaponin Ⅵ,chonglousaponin Ⅱ and chonglousaponin Ⅰ in yunnan hongyao capsules

投稿时间：2016-08-19

DOI：

中文关键词: 云南红药胶囊三七皂苷R1 重楼皂苷Ⅶ 重楼皂苷Ⅵ 重楼皂苷Ⅱ 重楼皂苷Ⅰ 高效液相色谱法

英文关键词: Yunnan hongyao capsules Notoginsenoside R₁ Chonglousaponin Ⅶ Chonglousaponin Ⅵ Chonglousaponin Ⅱ Chonglousaponin Ⅰ HPLC

基金项目:

作者	单位
张铭	辽宁中医药大学附属医院,辽宁沈阳 110032
袁杰	辽宁中医药大学附属医院,辽宁沈阳 110032
张新艳	辽宁省军区沈阳第一干休所卫生所,辽宁沈阳 110031

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中文摘要:

目的建立高效液相色谱法(HPLC)同时测定云南红药胶囊中三七皂苷R₁、重楼皂苷Ⅶ、重楼皂苷Ⅵ、重楼皂苷Ⅱ和重楼皂苷Ⅰ的含量,为云南红药胶囊质量的全面评价提供了科学依据。方法采用Diamonsil C₁₈ 柱(250 mm×4.6 mm,5 μm),以乙腈(A)-水(B)为流动相,进行梯度洗脱(0～12 min,18.0%A；12～19 min,18.0%A→25.0%A;19～27 min,25.0%A→45.0%A;27～34 min,45.0%A→55.0%A;34～40 min,55.0%A→18.0%A),检测波长为203 nm。流速0.9 mL·min-1,柱温30 ℃,进样量为10 μL。结果三七皂苷R1、重楼皂苷Ⅶ、重楼皂苷Ⅵ、重楼皂苷Ⅱ和重楼皂苷Ⅰ5个成分分别在7.32～146.40、4.70～94.00、3.85～77.00、6.97～139.40、11.09～221.80 mg·L-1内与色谱峰峰面积呈良好的线性关系；平均加样回收率均在97.12%～99.86%,RSD≤1.37%(n＝6)。结论该研究建立的HPLC法同时测定云南红药胶囊中的5个成分,样品处理方法简便,方法专属性强,测定结果准确,重复性好,为云南红药胶囊质量的全面评价提供了科学依据。

英文摘要:

Objective To establish an HPLC method for determination of notoginsenoside R₁,chonglousaponin Ⅶ,chonglousaponin Ⅵ,chonglousaponin Ⅱ and chonglousaponin Ⅰ,and to provide a scientific basis for the comprehensive quality evaluation of yunnan hongyao capsules.Methods The HPLC method was adopted with the Diamonsil C₁₈ column (250 mm × 4.6 mm,5 μm) and acetonitrile(A)-water (B) with gradient elution (0~12 min,18.0%A;12~19 min,18.0%A→25.0%A;19~27 min,25.0%A→45.0%A;27~34 min,45.0%A→55.0%A;34~40 min,55.0%A→18.0%A) as the mobile phase at the flow rate of 0.9 mL·min-1.The detection wavelength was 203 nm,the column temperature was set at 30 ℃,and sample quantity was 10 μL.Results The good linear relationships for notoginsenoside R₁,chonglousaponin Ⅶ,chonglousaponin Ⅵ,chonglousaponin Ⅱ and chonglousaponin Ⅰwere within the ranges of 7.32～146.40,4.70～94.00,3.85～77.00,6.97～139.40,1.09～221.80 mg·L-1,respectively.The average recoveries were between 97.12% and 99.86%,RSD≤1.37% (n=6).Conclusions An HPLC with gradient elution method has been successfully established for simultaneous determination of 5 components in yunnan hongyao capsules.The sample processing method is simple,specific,accurate,and repeatable,which provides a scientific basis for the comprehensive quality evaluation of yunnan hongyao capsules.

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