| 谢迪,李成网,宫鹏,等.高效液相色谱法同时测定复方瘤清合剂中莪术二酮、莪术醇、牻牛儿酮含量[J].安徽医药,2016,20(12):2262-2265. |
| 高效液相色谱法同时测定复方瘤清合剂中莪术二酮、莪术醇、牻牛儿酮含量 |
| Simultaneous determination of curdione,curcumenol and germacrone in compound liuqing mixture by HPLC |
| 投稿时间：2016-09-06 |
| DOI： |
| 中文关键词: 复方瘤清合剂 莪术二酮 莪术醇 牻牛儿酮 高效液相色谱法 含量测定 |
| 英文关键词: Compound liuqing mixture Curdione Curcumenol Germacrone HPLC Content determination |
| 基金项目: |
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| 摘要点击次数: 2290 |
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| 中文摘要: |
| 目的 建立一种同时测定复方瘤清合剂中莪术二酮、莪术醇、牻牛儿酮含量的高效液相色谱法(HPLC)。方法 采用Kromasil KR60-5CN色谱柱(4.6 mm × 150 mm,5 μm),以乙腈-水(34∶66)为流动相,检测波长为203 nm,进样量10 μm,流速1 mL·min-1,柱温30 ℃。结果 莪术二酮在0.139 8～1.398 0 g·L-1浓度范围内进样量与峰面积线性关系良好(r= 0.999 8,n=6),平均回收率为99.75%,RSD为0.76%；莪术醇在0.030 4～0.304 0 g·L-1浓度范围线性关系良好(r= 0.999 8,n=6),平均回收率为99.52%,RSD为1.04%；牻牛儿酮在0.096 8～0.968 0 g·L-1浓度范围线性关系良好(r=0.999 8,n=6),平均回收率为99.67%,RSD为0.63%。结论 该方法可以同时测定莪术中3种有效成分含量,并且准确、方便、专属性强,可以很好地控制制剂的质量。 |
| 英文摘要: |
| Objective To establish a method for determining simultaneously curdione,curcumenol and germacrone in compound liuqing mixture.Methods Kromasil KR60-5CN column(4.6 mm×150 mm,5 μm) was used with the mobile phase of acetonitrile-water(34∶66) at a flow rate of 1.0 mL·min -1 and the detection wavelength of 203 nm.The sample size was 10μm,the flow rate was set at 1 mL·min-1 and the temperature of column was at 30 ℃.Results The linear detection range of curdione was within 0.139 8～1.398 0 g·L-1(r=0.999 8,n= 6),and the average recovery was 99.75% (RSD=0.76%) .The linear detection range of curcumenol was within 0.030 4～0.304 0 g·L-1(r=0.999 8,n=6),and the average recovery was 99.52% (RSD=1.04%) .The linear detection range of germacrone was 0.096 8～0.968 0 g·L-1(r=0.999 8,n=6),and the average recovery was 99.67% (RSD=0.63%).Conclusions Three sesquiterpenoids in zedoary could be determined simultaneously by this method which is convenient,rapid and accurate.It can be used for quality control. |
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