海马区胶质纤维酸性蛋白及含1-亚基N-甲基-D-天冬氨酸受体表达与七氟醚致老龄大鼠认知障碍相关性分析

张秋莹; 李立明; 胡同江; 张加强

文章摘要

张秋莹,李立明,胡同江,等.海马区胶质纤维酸性蛋白及含1-亚基N-甲基-D-天冬氨酸受体表达与七氟醚致老龄大鼠认知障碍相关性分析[J].安徽医药,2017,21(7):1194-1198.

海马区胶质纤维酸性蛋白及含1-亚基N-甲基-D-天冬氨酸受体表达与七氟醚致老龄大鼠认知障碍相关性分析

Correlation between the expressions of GFAP and NR1 in the hippocampus and sevoflurane-induced cognitive disorders in aged rats

投稿时间：2016-09-21

DOI：

中文关键词: 认知功能障碍老龄七氟醚胶质纤维酸性蛋白含1-亚基N-甲基-D-天冬氨酸受体

英文关键词:

基金项目:河南省2015科技发展计划(152300410163)

作者	单位
张秋莹	焦作煤业集团有限责任公司中央医院，河南焦作 454003
李立明	焦作煤业集团有限责任公司中央医院，河南焦作 454003
胡同江	焦作煤业集团有限责任公司中央医院，河南焦作 454003
张加强	河南省人民医院，河南郑州 450003

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中文摘要:

目的探讨海马区胶质纤维酸性蛋白(GFAP)及含1-亚基N-甲基-D-天冬氨酸受体(NR1)表达与七氟醚所致老龄大鼠认知障碍相关性。方法 60只SD大鼠随机分为七氟醚吸入组(n=40)、氧气吸入组(n=10)和对照组(n=10),七氟醚吸入组吸入2%七氟醚120 min,氧气吸入组吸入纯氧120 min,对照组在常规环境中活动120 min。对氧气吸入组和对照组进行Morris水迷宫实验,七氟醚吸入组分别于大鼠苏醒后2 h(T₀)、24 h(T₁)、7 d(T₂)和14 d(T₃),随机选取10只大鼠进行水迷宫实验,测定逃避潜伏期和游泳总路程；采用免疫组化法和Western Blot法检测海马组织GFAP和NR1阳性表达情况,并分析GFAP和NR1阳性表达与水迷宫实验结果相关性。结果七氟醚吸入组在T0-2时逃避潜伏期和游泳总路程均较对照组和氧气吸入组延长,均差异有统计学意义(P<0.05)；七氟醚吸入组T0-2时刻CA1区和CA3区GFAP蛋白阳性表达均高于对照组和氧气吸入组,NR1蛋白阳性表达均低于对照组和氧气吸入组,均差异有统计学意义(P<0.05)；Western Blot检测显示,T0-2 时刻,七氟醚吸入组海马组织中GFAP蛋白表达均高于对照组和氧气吸入组,NR1蛋白表达均低于对照组和氧气吸入组,均差异有统计学意义(P<0.05)；Pearson相关分析显示,GFAP蛋白表达与大鼠逃避潜伏期和游泳总路程均呈正相关(r=0.628和0.728,P<0.001),而NR1蛋白表达则均呈负相关(r=-0.697和-0.735,P<0.001)。结论七氟醚吸入老龄大鼠出现了一过性认知障碍,与海马区星形胶质细胞活化指标GFAP表达上调和学习记忆通路受体亚单位NR1表达受抑制有关。

英文摘要:

Objective To investigate the correlation between GFAP and NR1 expression in the hippocampus and sevoflurane-induced cognitive disorders in aged rats. Methods Sixty SD rats were randomly assigned into the sevoflurane group (n=40),oxygen inhaled group (n=10) and control group (n=10).Rats in the sevoflurane group were inhaling 2% sevoflurane 120 min,and in oxygen inhaled group were inhaling pure oxygen 120min,while in the control group were staying in normal environment 120min.Rats in the oxygen inhaled group and control group were taking Morris water maze test.After awaking 2 h (T₀),24 h (T₁),7 d (T₂) and 14 d (T₃),each 10 rats in the sevoflurane group were randomly selected to take water maze test.The total escaping latency and total swimming distance were measured.The expressions of GFAP and NR1 in the hippocampus regions were detected by immunohistochemical and Western blot.The correlations between the expressions of GFAP and NR1 and water maze test results were analyzed. Results At T0-2,the escaping latency times and total swimming distances in the sevoflurane group were longer than the control group and oxygen inhaled group;the differences were statistically significant (P<0.05).At T0-2,the positive expression rates of GFAP in CA1 and CA3 regions in the sevoflurane group were higher than the control group and oxygen inhaled group,while the positive expression rates of NR1 were lower than the control group and oxygen inhaled group;the differences were statistically significant (P<0.05).Western blot analysis showed,at T0-2,the relative expression levels of GFAP proteins in hippocampus in the sevoflurane group were higher than the control group and oxygen inhaled group,while the relative expression levels of NR1 proteins were lower than the control group and oxygen inhaled group;the differences were all statistically significant (P<0.05).Pearson correlation analysis showed the expressions of GFAP were positively correlated with the total escaping latency times and total swimming distance (r=0.628 and 0.728,P<0.001),whereas,the expressoins of NR1 proteins were negatively correlated with the total escapinglatency times and swimming distance (r=-0.697 and -0.735,P<0.001). Conclusions Sevoflurane inhaling could result in a transient cognitive impairment in aged rats.It might be related to the up-regulated expressions of GFAP,which was activation marker for astroglial cell,and the inhibited expressions of NR1,which was accessing receptor for learning and memory in the hippocampus.

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