| 徐亚运,陈征,葛金芳,等.氟西汀对皮质酮诱导Nesfatin-1含量升高的影响[J].安徽医药,2017,21(8):1380-1383. |
| 氟西汀对皮质酮诱导Nesfatin-1含量升高的影响 |
| Effect of fluoxetine on the increased content of Nesfatin-1 induced by corticosterone in the SH-SY5Y cell |
| 投稿时间：2017-01-16 |
| DOI： |
| 中文关键词: 皮质酮 氟西汀 Nesfatin-1 神经母细胞瘤SH-SY5Y |
| 英文关键词: |
| 基金项目:国家自然科学基金(81401122) 作者简介:徐亚运,男,硕士研究生 |
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| 中文摘要: |
| 目的 观察氟西汀对皮质酮诱导的SH-SY5Y细胞株中下丘脑摄食调节因子Nesfatin-1含量升高的影响。方法 采用体外培养细胞的方法,建立SH-SY5Y细胞皮质酮损伤模型,设氟西汀给药组(10-9～10-5 mol·L-1)、皮质酮组和阴性对照组,采用实时荧光定量PCR、Western blot和免疫组化的方法观察细胞中Nesfatin-1 mRNA及蛋白表达的变化。结果 与对照组相比,皮质酮组细胞Nesfatin-1 mRNA和蛋白含量均显著升高；氟西汀(10-9～10-5 mol·L-1)处理后Nesfatin-1 mRNA和蛋白含量均显著降低。结论 氟西汀体外用药能够逆转皮质酮诱导的SH-SY5Y细胞Nesfatin-1表达上调。 |
| 英文摘要: |
| Objective To investigate the effect of fluoxetine on the increased content of Nesfatin-1 induced by corticosterone in the human neuroblastoma SH-SY5Y cell. Methods Using SH-SY5Y cell line to establish corticosterone-induced SH-SY5Y cell injury model.We designed five groups of fluoxetine (10-9-10-5 mol·L-1),corticosterone group and control group.Real-time PCR,western blot and immunohistochemistry were used to measure the mRNA and protein expressions of Nesfatin-1. Results The mRNA and protein expressions of Nesfatin-1 in the corticosterone group were higher than those of the control group.The mRNA and protein expressions of Nesfatin-1 in the fluoxetine group were lower than those of the corticosterone group. Conclusion The increased content of Nesfatin-1 induced by the corticosterone treatment could be decrease by the fluoxetine treatment. |
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