| 蔡颖,商玉萍,高学坤.苹果多酚对脂多糖刺激的RAW264.7细胞COX-2/PGE2和iNOS/NO表达的研究[J].安徽医药,2017,21(11):1975-1978. |
| 苹果多酚对脂多糖刺激的RAW264.7细胞COX-2/PGE2和iNOS/NO表达的研究 |
| Effect of apple polyphenols on COX-2/PGE2 and iNOS/NO expression in LPS-stimulated RAW264.7 cells |
| 投稿时间：2016-10-19 |
| DOI： |
| 中文关键词: 苹果多酚 脂多糖 抗炎活性 |
| 英文关键词: Apple polyphenols extract LPS Anti-inflammatory activity |
| 基金项目: |
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| 摘要点击次数: 2744 |
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| 中文摘要: |
| 目的 研究苹果多酚(APE)对脂多糖(LPS)诱发的RAW264.7细胞炎症COX-2/PGE2和iNOS/NO表达的抑制作用。方法 采用APE干预LPS刺激的小鼠单核/巨噬细胞RAW264.7,然后用Griess Reagent法和ELISA法检测细胞分泌的一氧化氮(NO)和前列腺素E2(PGE2)的水平,并且采用RT-qPCR和Western blotting技术检测APE对诱导型一氧化氮合酶(iNOS)和环氧化酶-2(COX-2)水平的影响以及核转录因子(NF-κB)的蛋白表达。结果 APE能显著抑制LPS刺激的RAW264.7细胞中NO、PGE2的含量,下调iNOS及COX-2的表达及NF-κB的磷酸化。结论 APE通过下调NF-κB的活性及iNOS与COX-2表达而发挥抗炎作用。 |
| 英文摘要: |
| Objective To investigate the effect of apple polyphenols on the expression of iNOS/NO and COX-2/PGE2 in LPS-stimulated RAW264.7 cells. Methods RAW264.7 cells were stimulated by LPS and treated with apple polyphenols.NO and PEG2 levels in supernatant were detected by Griess Reagent and ELISA,and the expression of iNOS and COX-2 were evaluated by RT-qPCR and Western blotting. Results Apple polyphenols strongly inhibited the production of NO,PEG2,and the expression of iNOS,COX-2 and NF-κB. Conclusions Apple polyphenols exert anti-inflammatory effect through down-regulation of NF-κB activity and the expression of iNOS and COX-2. |
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