| 王金艳,孟祥茹,肖亚利,等.熊果酸对动脉粥样硬化大鼠氧化应激和炎性反应的影响[J].安徽医药,2018,22(5):815-818. |
| 熊果酸对动脉粥样硬化大鼠氧化应激和炎性反应的影响 |
| The effects and mechanism of ursolic acid on oxidative stress and inflammation in atherosclerosis rats |
| 投稿时间：2016-08-18 |
| DOI： |
| 中文关键词: 熊果酸 动脉粥样硬化 氧化应激 炎症 机制 |
| 英文关键词: ursolic acid atherosclerosis oxidative stress inflammation mechanism |
| 基金项目: |
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| 摘要点击次数: 1905 |
| 全文下载次数: 496 |
| 中文摘要: |
| 目的 研究熊果酸(UA)对动脉粥样硬化(AS)大鼠氧化应激和炎性反应的抑制作用,并探讨其机制。方法 采用高脂饲料喂养结合腹腔注射VD3的方法建立早期AS大鼠模型,并分别灌胃给予UA(10、20、40 mg·kg-1·d-1)和辛伐他汀(SV,1.8 mg·kg-1·d-1)进行干预,作为干预组,另设模型组和健康对照组(喂食正常饲料并腹腔注射生理盐水)。采用比色法测定血清中抗氧化酶[超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)、过氧化氢酶(CAT)]活性和丙二醛(MDA)、活性氧簇(ROS)含量；酶联免疫法(ELISA)测定血浆中炎性细胞因子[C反应蛋白(CRP)、肿瘤坏死因子-α(TNF-α)、白细胞介素-1β(IL-1β)、白细胞介素-6(IL-6)、白细胞介素-10(IL-10)]含量；苏木精-尹红(HE)染色观察主动脉形态结构改变并进行病变分级。结果 UA(20、40 mg·kg-1·d-1)同步干预组大鼠血清中SOD、CAT活性较模型组显著升高[(318.3±29.4)、(12.3±2.4) U·mL-1 vs(249.3±25.1)、(9.3±1.7) U·mL-1],MDA、ROS含量显著降低[(31.2±5.1) vs(49.6±7.0) mmol·L-1和(291.3±31.8) vs(402.9±35.7) U·mL-1],且UA 40 mg·kg-1·d-1干预组GSH-Px活性显著升高[(809.3±58.4) vs(676.1±48.5) U·mL-1]；UA(20、40 mg·kg-1·d-1)同步干预组大鼠血浆中CRP、TNF-α、IL-1β、IL-6含量较模型组均显著降低,且UA 40 mg·kg-1·d-1干预组IL-10含量显著升高[(37.6±4.9) vs(23.9±3.2) ng·L-1]；UA同步干预组大鼠主动脉形态结构改变较模型组明显改善、病变评分显著降低,其中以UA 40 mg·kg-1·d-1干预组效果最为显著。结论UA可能通过抑制氧化应激损伤和炎性反应而对AS大鼠起到一定保护作用。 |
| 英文摘要: |
| Objective To investigate the effects and mechanism of Ursolic Acid (UA) on oxidative stress and inflammation in atherosclerosis rats.Methods The rat models were made by dirtying with high fat and injecting VD3,and at the same time,Intervention group was treated with UA (10,0,40 mg·kg-1·d-1) and simvastatin (SV),(1.8 mg·kg-1·d-1);setting control group and model group.The activity of SOD,GSH-Px,CAT and the content of MDA,ROS in serum were determined;the level of CRP,TNF-α,IL-1β,IL-6,IL-10 in serum were determined;the histopathological changes of abdominal aorta was observed by HE staining and graded.Results Serum SOD and CAT activity in UA (20 mg·kg-1·d-1) intervention group were significantly higher than those in the model group [(318.3±29.4) vs (249.3±25.1),(12.3±2.4) U·mL-1 vs (9.3±1.7) U·mL-1, respectively], and MDA and ROS levels were significantly reduced [(31.2±5.1) vs (49.6±7.0) mmol·L-1,(291.3±31.8) vs (402.9±35.7) U·mL-1] The activity of GSH-Px in UA (40 mg·kg-1·d-1) intervention group was significantly increased [(809.3±58.4) vs (676.1±48.5) U·mL-1].Plasma CRP,TNF-α,IL-1β,IL-6 levels in UA (20,0 mg·kg-1·d-1) intervention group were significantly decreased compared with model group,and the IL-10 level in the UA (40 mg·kg-1·d-1) intervention group was significantly increased [(37.6±4.9) vs (23.9±3.2) ng·L-1].Compared with model control group, the histopathological changes in UA intervention groups were significantly improved,and the lesion score was significantly reduced,with the effect of UA (40 mg·kg-1·d-1) intervention group being most significant.Conclusions UA has protective effects on the rats with atherosclerosis perhaps through depressing oxidative stress and inflammation. |
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