| 谢强,汪世存.固相萃取合成PET-CT肿瘤探针2-[18F]-2-脱氧-β-D-葡萄糖及其影响因素初探[J].安徽医药,2018,22(7):1241-1244. |
| 固相萃取合成PET-CT肿瘤探针2-[18F]-2-脱氧-β-D-葡萄糖及其影响因素初探 |
| Solid-phase extraction synthesis PET-CT tumor probe 2-[18F]-2-deoxidation-β-D-glucose and its influencing factors |
| 投稿时间：2017-09-28 |
| DOI： |
| 中文关键词: 正电子发射断层扫描及电脑断层扫描 3-O-甲基葡萄糖 固相萃取 工艺学, 制药 |
| 英文关键词: Positron-emission tomography and computed tomography 3-O-Methylglucose Solid phase extraction Technology, pharmaceutical |
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| 摘要点击次数: 2048 |
| 全文下载次数: 593 |
| 中文摘要: |
| 目的 探讨常见肿瘤显像剂2-[18F]-2-脱氧-β-D-葡萄糖([18F]-FDG)在西门子Explora One化学合成模块上全自动合成方法、质量控制以及影响因素。方法 西门子Eclipse RD回旋加速器生产出[18F-]与以三氟甘露糖为前体进行亲核取代反应合成乙酰化[18F]-FDG,高纯氮气推动,Sep-Pak C18萃取柱吸附中间体,氢氧化钠水解C-18柱上中间产物,经纯化,灭菌最终得到[18F]-FDG注射液。通过薄层色谱法对合成的产品进行放射化学纯度(RCP)检测,肿瘤患者行[18F]-FDG PET-CT扫描。 结果 最终合成产物[18F]-FDG的RCP>95%,20 mg的三氟甘露糖可获得未校正合成效率约为60%。结论 使用 ABX [18F]-FDG套件,Explora One 化学合成模块实现固相萃取法合成PET显像剂[18F]-FDG,合成时间短,稳定快捷。 |
| 英文摘要: |
| Objective To present a method for automatic synthesis of common tumor imaging agents [18F]-FDG by Explora One module from Siemens corporation, and discuss the method for quality control, factors that affect synthesis of the tracers. Methods Siemens Eclipse RD cyclotron firstly produced [18F-] and interacted with a precursor (1,3,4,6-4-O-acetyl-2-O-trifluorosulfonyl-beta-D-pyranose) of [18F]-FDG. Acetylated-[18F]-FDG was pushed with high purity nitrogen and the intermediates (acetylated-[18F]-FDG) captured by Sep-Pak C18 extraction column. NaOH hydrolysis the intermediate carried by C-18 column, after purification, sterilization and eventually get [18F]-FDG injection. The radiochemical purity (RCP) of the synthesized products was detected by TLC. PET-CT scan of [18F]-FDG tumor patient was carried out and confirmed by pathology. Results The RCP of [18F]-FDG is over 95%. 20 mg of trifluorocarbon can achieve an uncorrected synthetic efficiency of about 60%. Conclusions It is easily and steadily to automatically synthesis the tumor imaging agents [18F]-FDG by using ABX [18F]-FDG cassette with Explora One module. The synthesis time was short, stable and fast. |
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