| 赵鹏,李树锋.葡萄糖转运蛋白-1的表达对骨肉瘤细胞生物学特征的影响[J].安徽医药,2018,22(7):1273-1277. |
| 葡萄糖转运蛋白-1的表达对骨肉瘤细胞生物学特征的影响 |
| Effect of the expression of glucose transporter-1 on the biological characteristics of osteosarcoma cells |
| 投稿时间：2017-01-19 |
| DOI： |
| 中文关键词: 骨肉瘤 葡萄糖转运体1型 RNA, 小分子干扰 细胞增殖 肿瘤侵润 |
| 英文关键词: Osteosarcoma Glucose transporter type 1 RNA, small interfering Cell proliferation Neoplasm invasiveness |
| 基金项目:山东省自然科学基金面上项目(ZR2014HM049)葡萄糖转运蛋白-1的表达对骨肉瘤细胞生物学特征的影响赵鹏1,李树锋2 (1. 焦作煤业集团中央医院,河南 焦作 454000；2. 山东省千佛山医院,山东 济南 250014) |
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| 中文摘要: |
| 目的 探讨葡萄糖转运蛋白-1(GLUT1)在骨肉瘤组织中的表达及干扰其表达对骨肉瘤细胞生物学特征的影响。方法 选取择期行手术治疗的初诊骨肉瘤患者57例,利用实时荧光定量PCR技术检测骨肉瘤及癌旁组织中GLUT1基因表达；培养人骨肉瘤MG63细胞,根据转染物不同分为siRNA-GLUT1组、siRNA-NC组和空白对照组,利用实时荧光定量PCR技术和 蛋白质印迹法分别检测各转染组细胞中GLUT1基因和蛋白表达,MTT法检测各转染组细胞增殖能力,利用流式细胞技术检测各转染组细胞凋亡情况,Transwell法检测各转染组细胞迁移和侵袭能力。结果 骨肉瘤组织中GLUT1 mRNA相对表达量1.95±0.18,显著高于癌旁组织的1.27±0.13,差异有统计学意义(P<0.05)；骨肉瘤组织中GLUT1 mRNA相对表达量与临床分期、淋巴结转移和肺部转移有关(P<0.05)；MTT检测结果显示,siRNA-GLUT1组细胞吸光度A值在24、48、72、96 h时均低于siRNA-NC组和空白对照组,均差异有统计学意义(P<0.05)；流式细胞检测结果显示,siRNA-GLUT1组细胞凋亡率(25.7±2.6)%,显著高于siRNA-NC组(6.3±1.4)%和空白对照组(6.9±1.7)%,差异有统计学意义(P<0.05)；siRNA-GLUT1组迁移细胞数和侵袭细胞数低于siRNA-NC组和空白对照,均差异有统计学意义(P<0.05)。结论 GLUT1在骨肉瘤组织中呈高表达,特异性抑制GLUT1基因表达可有效抑制人骨肉瘤MG63细胞增殖,加速细胞凋亡,减少细胞迁移及侵袭。 |
| 英文摘要: |
| Objective To investigate the expression of glucose transporter-1 (GLUT1) in osteosarcoma tissues and effect of interfering its expression on the biological characteristics of osteosarcoma cells. Methods 57 patients with newly diagnosed osteosarcoma undergoing elective surgery were selected. The expressions of GLUT1 genes in osteosarcoma and adjacent tissues were detected by real-time fluorescence quantitative PCR. Human osteosarcoma MG63 cells were cultured. According to the different transfection, the cells were divided into siRNA-GLUT1 group, siRNA-NC group and blank control group. The expressions of GLUT1 genes and proteins in the different transfected groups were detected by using real-time fluorescent quantitative PCR and Western blot, respectively. The cell proliferation abilities in the different transfected groups were detected by using MTT. The apoptosis of cells in the different transfected groups were detected by flow cytometry. The migration and invasion abilities of cells in the different transfected groups were detected by Transwell method. Results The relative expression levels of GLUT1 mRNA in the osteosarcoma tissues were 1.27±0.13, which were significantly higher than 1.95±0.18 in the adjacent tissues, the difference was statistically significant (P<0.05). The relative expression levels of GLUT1 mRNA in osteosarcoma tissues were related with clinical stage, lymph node metastasis and lung metastasis (P<0.05). MTT assay showed that the absorbance Avalues in the siRNA-GLUT1 group at 24,8, 72,6 h were significantly lower than those in the siRNA-NC group and blank control group (P<0.05). The apoptosis rate in the siRNA-GLUT1 group was (25.7±2.6)%, which was significantly higher than those in the siRNA-NC group and blank control group, which were (6.3±1.4)% and (6.9±1.7)%, respectively, the difference was statistically significant (P<0.05). The numbers of migrating cells and invasion cells in siRNA-GLUT1 group were lower than those in the siRNA-NC group and blank control group (P<0.05). Conclusion GLUT1 was highly expressed in osteosarcoma tissue. Specific inhibition of GLUT1 gene expression could effectively inhibit the proliferation of human osteosarcoma MG63 cells, accelerate cell apoptosis, and reduce cell migration and invasion. |
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