| 华燕青,张楠.超高效液相色谱法测定垂盆草颗粒中3种黄酮成分的含量[J].安徽医药,2018,22(8):1457-1459. |
| 超高效液相色谱法测定垂盆草颗粒中3种黄酮成分的含量 |
| Determination of three flavonoids components in chuipencaokeli by UPLC |
| 投稿时间：2017-03-06 |
| DOI： |
| 中文关键词: 超高效液相色谱法 垂盆草颗粒 含量测定 槲皮素 山柰素 异鼠李素 |
| 英文关键词: UPLC Chuipencaokeli Content Determination Meletin Kaempferide isorhamnetin |
| 基金项目:杨凌职业技术学院科学研究项目(A2015049)◇药物分析◇超高效液相色谱法测定垂盆草颗粒中3种黄酮成分的含量华燕青1,张楠2 (1.杨凌职业技术学院,陕西 杨凌 712100;2.西安市食品药品检验所,陕西 西安 710065) |
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| 中文摘要: |
| 目的 建立超高效液相色谱法(UPLC)同时测定垂盆草颗粒中槲皮素、山柰素与异鼠李素的含量。方法 色谱柱Thermo Scientific AcclaimTM RSLC 120 C18 (2.1 mm×100 mm,2.2 μm),流动相为甲醇-0.4%磷酸(50 ∶50),柱温为30 ℃,流速为0.3 mL·min-1,检测波长为360 nm, 进样量1 μL。结果 槲皮素、山柰素与异鼠李素的线性范围分别为:50~1 000 μg, 20~400 μg, 20~400 μg,且与峰面积呈良好线性关系(r=0.999 9,0.999 9,0.999 6),精密度、稳定性、重复性试验的RSD均<2%,平均加样回收率(n=9)分别为99.35%,99.38%,98.91%,RSD分别为1.04%、0.98%、1.01%。结论 该法高效、准确、灵敏,可作为测定垂盆草颗粒中槲皮素、山柰素与异鼠李素含量的有效方法。 |
| 英文摘要: |
| Objective To develop a method for the determination of three flavonoids components in chuipencaokeli by UPLC. Methods Thermo Scientific AcclaimTM RSLC 120 C18 chromatographic column (2.1 mm×100 mm, 2.2 μm) was used, with methanol-0.4% phosphoric acid (50 ∶50) as the mobile phase with the flow rate of 0.3 mL·min-1. Detection wavelength was 360 nm, column temperature was 30℃and injection volume was 1 μL.Results The linear range was 50-1 000 μg for meletin, 20-400 μg for kaempferide, 20-400 μg for isorhamnetin.There was a good linear relationship. The RSDs of precision, stability and repeatability tests were less than 2.0％ respectively. The average recovery was 99.35%, 99.38%,98.19% (RSD<2%). Conclusion The method is rapid, accurate and effective. It is reliable method for determination of three flavonoids in chuipencaokeli, which provide a basis for quality standards. |
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