| 汪俊,王芳,史亚男.T淋巴瘤侵袭转移诱导因子1在子宫颈癌组织中表达及意义[J].安徽医药,2018,22(9):1689-1693. |
| T淋巴瘤侵袭转移诱导因子1在子宫颈癌组织中表达及意义 |
| Expression and significance of Tlymphoma invasion and metastasis inducing factor 1 in cervical cancer |
| 投稿时间：2017-04-04 |
| DOI： |
| 中文关键词: 子宫颈癌 T淋巴瘤侵袭转移诱导因子1 预后 |
| 英文关键词: Cervical cancer T lymphoma invasion and metastasis inducing factor 1 Prognosis |
| 基金项目:广东省自然科学基金(2014A030310149)T淋巴瘤侵袭转移诱导因子1在子宫颈癌组织中表达及意义汪俊1,王芳1,史亚男2 (1.武汉科技大学附属汉阳医院,湖北 武汉 430000；2.中山大学第一附属医院,广东 广州 510000) |
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| 中文摘要: |
| 目的 探讨T淋巴瘤侵袭转移诱导因子1(Tiam1)在子宫颈癌组织中表达及意义。方法 留取子宫颈癌组织84例,宫颈上皮内瘤变(CIN)组织56例,留取子宫良性病变患者正常宫颈组织43例,利用实时荧光定量PCR技术检测不同组织中Tiam1基因表达,利用免疫组织化学法检测不同组织中Tiam1蛋白表达。结果 子宫颈癌组织中Tiam1 mRNA相对表达量和蛋白阳性表达率[(2.05±0.17)%和82.1%]均高于CIN组织[(1.74±0.24)%和46.4%]和正常宫颈组织[(1.27±0.16)%和14.0%],且CIN组织高于正常宫颈组织(P<0.001),CIN Ⅰ组织中Tiam1 mRNA相对表达量(1.54±0.19)显著低于CIN Ⅱ～Ⅲ组织(1.81±0.23),差异有统计学意义(t=22.186,P=0.016),即子宫颈癌组织> CIN Ⅱ～Ⅲ组织> CIN Ⅰ组织>正常宫颈组织；子宫颈癌组织中Tiam1 mRNA相对表达量和蛋白阳性表达率均与临床分期、病理分级、浸润深度、淋巴结转移有关(P<0.05)；Kaplan-Meier生存分析结果显示,高表达组患者生存时间44.13个月,低表达组患者生存时间61.00个月,Tiam1蛋白阳性表达患者生存时间34.67个月,阴性表达患者生存时间63.76个月,Log-Rank检验显示均差异有统计学意义(均P<0.05)；Cox比例风险回归模型分析结果显示,临床分期、淋巴结转移和Tiam1表达是患者预后的影响因素(均P<0.05)。结论 Tiam1基因和蛋白在子宫颈癌组织中呈高表达,且与患者预后密切相关,有望成为预测患者预后的有效分子指标。 |
| 英文摘要: |
| Objective To investigate the expression and significance of Tlymphoma invasion and metastasis inducing factor 1 (Tiam1) in cervical cancer.Methods 84 cases of cervical cancer tissues and 56 cases of cervical intraepithelial neoplasia (CIN) tissues were selected.43 cases of normal cervical tissues from patients with benign uterine disease were selected.The expressions of Tiam1 genes in different tissues were detected by using real-time fluorescence quantitative PCR.The expressions of Tiam1 proteins in different tissues were detected by using immunohistochemistry.Results The relative expression level of Tiam1 mRNA and positive expression rate of Tiam1 protein in cervical cancer tissues (2.05±0.17 and 82.1 %) were higher than the CIN tissues (1.74±0.24 and 46.4 %) and normal cervical tissues (1.27±0.16 and 14.0 %), The CIN tissues were higher than the normal cervical tissues (P<0.001). The relative expression level of Tiam1 mRNA in CIN Ⅰ tissues was (1.54±0.19),which was significantly lower than that in CIN Ⅱ-Ⅲ tissues,which was (1.81±0.23),and the difference was statistically significant (t=22.186,P= 0.016),that was,cervical cancer tissue>CIN Ⅱ-Ⅲ tissue>CIN Ⅰ tissue>normal cervical tissue.The relative expression level of Tiam1 mRNA and protein positive expression rate in cervical cancer tissues was correlated with clinical stage,pathological grade,depth of invasion and lymph node metastasis (P<0.05).Kaplan-Meier survival analysis showed that the survival time of patients in the high expression group was 44.13 months,and the survival time of patients in the low expression group was 61.00 months,the survival time of patients with Tiam1 protein positive expression was 34.67 months,and the survival time of patients with Tiam1 protein negative expression was 63.76 months,Log-rank test showed the differences were statistically significant(P<0.05).Cox proportional hazards regression model analysis results showed that clinical stage,lymph node metastasis and Tiam1 expression were the influencing factors for prognostic of patients (P<0.05).Conclusion Tiam1 gene and protein are highly expressed in cervical cancer tissues,and closely related to the prognosis of patients.It is expected to be an effective molecular marker for predicting the prognosis of patients. |
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