| 丁露,夏云红,王为民,等.CD8阳性T细胞亚群在肺癌外周血的检测及分析[J].安徽医药,2018,22(10):1926-1928. |
| CD8阳性T细胞亚群在肺癌外周血的检测及分析 |
| Detection and analysis of CD8+ T lymphocyte subets in peripheral blood of patients with lung cancer |
| 投稿时间：2017-01-15 |
| DOI： |
| 中文关键词: 肺肿廇 CD8阳性T淋巴细胞 抗原,CD28 抗原,CD57 |
| 英文关键词: Lung neoplasms CD8-positive T-lymphocytes Antigens,CD28 Antigens,CD57 |
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| 中文摘要: |
| 目的 检测肺癌患者外周血清中CD8+T细胞亚群CD28+及CD57+的表达程度,探讨其临床意义。 方法 合肥市第二人民医院经细胞学或病理学诊断为肺癌的初次治疗患者30例, TNM分期(UICC,第7版)Ⅲ期12例,Ⅳ期18例。病理类型腺癌15例,鳞癌8例,小细胞肺癌7例。流式细胞仪分别检测肺癌患者组治疗前后及健康对照组人群的血清中CD8+T细胞CD28+和CD57+的表达水平,分析该表达水平变化及与肺癌临床分期和病理学类型之间的关系。 结果 肺癌患者治疗前血清中CD28+T细胞亚群比例为(12.34±3.72)%,CD57+ T细胞亚群比例为(19.53±5.26)%。而健康对照组血清中CD28+T细胞亚群比例为(23.53±4.15)%,CD57+ T细胞亚群比例为(11.37±2.73)%,差异有统计学意义(P<0.05),该类指标与肿瘤病理类型无关,而随着不同临床分期增加,CD8+CD57+T细胞未见差异,而CD8+CD28+T细胞表达差异有统计学意义(P<0.05)。经两周期化疗后,缓解组(完全缓解+部分缓解)患者血清中CD28+、CD57+T细胞亚群比与健康对照组患者相比,差异无统计学意义(P>0.05),而未缓解组(稳定+进展)患者的CD28+、CD57+T细胞亚群比例分别为(10.62±2.74)%、(20.45±4.36)%.与健康对照组差异有统计学意义(P<0.05)。 结论 肺癌患者血清中CD8+CD28+T细胞表达较正常人群明显减低,而CD8+CD57+T细胞表达较正常人群明显升高,联合该两种T细胞亚群检测不仅可以了解肺癌患者免疫状况,同时可以评估肺癌患者预后,有一定临床应用价值。 |
| 英文摘要: |
| Objective To detect and analyze the expression levels of CD28+ and CD57+,and subsets of CD8+ T cells,in peripheral blood of patients with lung cancer as well as to explore the clinical significance. Methods A total of 30 initially treated patients cytologically or pathologically diagnosed as lung cancer in The Second People′s Hospital of Hefei were enrolled in the study,including 12 cases of stage III and 18 cases of stage IV according to TNM system (UICC,the 7th edition) or 15 cases of glandular cancer,8 cases of squamous cancer and 7 cases of small cell lung cancer according to pathology types.The expression levels of CD28+ and CD57+ in healthy subjects and patients with lung cancer before and after treatment were tested by flow cytometry.The correlation between expression alteration and clinical stages as well as pathology types of lung cancer was studied. Results Compared with healthy subjects,there was significant differences in ratio of CD28+/CD57+ [(12.34±3.72)% vs. (23.53±4.15)%,(19.53±5.26)% vs. (11.37±2.73)%,respectively; P<0.05],and CD28+/CD57+ levels were not associated with pathology types.There was no difference in CD8+CD57+ T cells with the increase of different clinical stages, and the expression of CD8+CD28+ T cells was statistically significant (P<0.05).Compared with healthy subjects,after 2-weekchemotherapy,the ratio of CD28+/CD57+ in response arm (complete response and partial response) was not significantly different (P>0.05),while that in non response arm (stable disease and advanced disease) was significantly different [(10.62±2.74)% vs. (20.45±4.36)%,P<0.05]. Conclusions Compared with healthy subjects,the expression level of CD8+CD28+ in serum of patients with lung cancer is significantly lower while the expression level of CD8+CD57+ is significantly higher in patients with lung cancer.The combination of these two Tcell subsets is helpful to effectively evaluate the immunity and prognosis of patients with lung cancer,and has certain clinical application value. |
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