| 韩涛,曹明.乳腺癌组织miR-20a的表达及其对乳腺癌细胞自噬的影响[J].安徽医药,2018,22(10):1936-1939. |
| 乳腺癌组织miR-20a的表达及其对乳腺癌细胞自噬的影响 |
| Expression of miR-20a in breast cancer tissues and its effect on breast cancer cell autophagy |
| 投稿时间：2018-02-28 |
| DOI： |
| 中文关键词: 乳腺肿瘤 自噬 微RNAs 逆转录聚合酶链反应 蛋白质印迹 |
| 英文关键词: Breast neoplasms Autophagy MicroRNAs Reverse transcriptase polymerase chain reaction Western blotting |
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| 中文摘要: |
| 目的 探究乳腺癌患者肿瘤组织中miR-20a的表达情况及其对乳腺癌细胞自噬功能的作用。 方法 收集芜湖市第一人民医院2016年1月至2017年12月间收治的接受手术治疗的乳腺癌患者56例,逆转录实时定量聚合酶链反应(qRT-PCR)检测肿瘤组织与癌旁组织miR-20a与自噬相关基因Beclin1、LC3、ATG5及ATG7 mRNA的表达水平。脂质体法转染pcDNA3/pri-miR-20a质粒至MCF7细胞,免疫蛋白印记实验检测Beclin 1与饥饿条件下LC3Ⅱ表达水平,免疫荧光实验检测自噬斑点。 结果 肿瘤组织miR-20a的平均表达水平为211.35±67.43,癌旁组织miR-20a的平均表达水平为126.72±24.37,差异有统计学意义(P<0.05)。此外,肿瘤组织较癌旁组织Beclin1 mRNA表达量降低(P<0.05),ATG5、ATG7与ATG12 mRNA的表达量差异无统计学意义 (P>0.05)。过表达miR-20a后MCF7细胞Beclin1蛋白表达量降低,饥饿刺激下LC3Ⅱ表达水平降低且自噬斑点减少。 结论 乳腺癌组织中miR-20a呈高表达,并且降低自噬调节蛋白Beclin1的表达以抑制自噬活化。 |
| 英文摘要: |
| Objective To investigate the expressions of miR-20b in breast cancer and its effect on autophagy in breast cancer cells in vitro. Methods Fifty and six cases of patients suffering from breast cancer who underwent surgical treatment in Wuhu First People′s Hospital during January 2016 and December 2017 were enrolled.Reverse transcription real-time quantitative polymerase chain reaction (qRT-PCR) was used to detect the expression of miR-20a,and autophagy associated gene mRNA including Beclin1,LC3,ATG5 and ATG7 in breast cancer tissues and adjacent tissues.pcDNA3/pri-miR-20a plasmid was transfected into MCF7 cells by lipofectamine 2000 reagent,and Beclin1 protein expression and LC3Ⅱexpression level under starvation conditions were detected by Western blot.The autophagy spots were observed by indirect immunofluorescence. Results The average levels of miR-20a in breast cancer tissues was(211.35±67.43),and the average levels of miR-20a in adjacent tissues was(126.72±24.37),and the difference was statistically significant (P<0.05).Besides,the expression of Beclin1 mRNA in breast cancer tissues was lower than that in adjacent tissues (P<0.05).There was no significant difference in the expression of ATG5,ATG7 and ATG12 mRNA between breast cancer tissues and adjacent tissues (P>0.05).After overexpression of miR-20a,the level of Beclin1 protein in MCF7 cells was decreased,the expression level of LC3Ⅱand the autophagy spots under starvation conditions were decreased. Conclusions The miR-20a expression in breast cancer tissue is high,overexpression of miR-20a down-regulates the expression of autophagy associated protein Beclin1to suppress autophagy activation. |
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