| 胡丽,饶婷,段丹,等.新型硫化氢供体GYY4137对大鼠肾缺血再灌注损伤的保护作用[J].安徽医药,2018,22(11):2092-2095. |
| 新型硫化氢供体GYY4137对大鼠肾缺血再灌注损伤的保护作用 |
| Protective effects of GYY4137,a novel hydrogen sulfide-releasing molecule on renal ischemia-reperfusion injury in rats |
| 投稿时间：2016-12-22 |
| DOI： |
| 中文关键词: GYY4137 缺血再灌注损伤 肾脏 |
| 英文关键词: GYY4137 Ischemia reperfusion injury Kidney |
| 基金项目: |
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| 中文摘要: |
| 目的 观察GYY4137对大鼠肾缺血再灌注损伤(IRI)后的保护作用。方法 45只健康雄性SD大鼠,按随机数字表法分成正常组(A组)、缺血再灌注组(B组)和缺血再灌注+GYY4137组(C组)。B组和C组大鼠建立肾脏缺血再灌注模型,C组在再灌注同时腹腔注射GYY4137(100 μmol·kg-1)。方法 苏木精和伊红(HE)染色观察肾脏组织病理改变；测定丙二醛(MDA)、超氧化物岐化酶(SOD)、血肌酐(Scr)和尿素氮(BUN)含量；采用原位缺口末端标记检测肾小管上皮细胞凋亡指数(AI)；反转录聚合酶链反应(RT-PCR)检测肾脏组织中的Bax,Bcl-2和caspase-3的表达情况。结果 B组可见肾小管扩张,肾小管上皮细胞坏死等表现,经GYY4137治疗后明显改善；A组,B组和C组的凋亡指数分别为(4.58±0.43)％、(23.52±1.87)％、(10.86±0.87)％,SOD活性分别为(235.43±5.94)、(115.38±3.79)、(162.39±3.92) U·mg-1,MDA含量分别为(1.27±0.28)、(3.64±0.53)、(2.33±0.57) nmol·mg-1,Scr分别为(63.26±5.21)、(287.64±10.32)、(106.47±6.29)μmol·L-1,BUN分别为(7.46±1.26)、(13.65±1.04)、(9.65±1.35) mmol·L-1；与A组比较,B组中Bax、caspase-3 mRNA表达水平明显增高,Bcl-2表达水平降低(P<0.05)。和B组比较,C组中Bcl-2表达水平增高和Bax、caspase-3表达减低(P<0.05)。结论 GYY4137可减轻氧化应激水平和抑制凋亡,从而在肾脏组织IRI过程中发挥保护作用。 |
| 英文摘要: |
| Objective To investigate the protective effect of GYY4137 on renal ischemia reperfusion injury in rats.Methods Forty-five male SD rats were randomly divided into three groups:normal group (group A),ischemia reperfusion group (group B) and ischemia reperfusion+GYY4137 group (group C).Renal ischemia reperfusion model was established in group Band C,GYY4137 (100 μmol·kg-1) was injected intraperitoneally followed reperfusion in group C.Methods The pathological changes of the kidney were observed by HE staining.MDA、SOD、Scrand BUN content were determined;Cell apoptosis index was detected by dUTP nick end labeling;The expression of Bax,Bcl-2 and caspase-3 in renal tissue was detected by RT-PCR.Results Group Bshowed renal tubular expansion,renal tubular epithelial cell necrosis,GYY4137 treatment significantly improved the above changes.AI in group A,group Band group Cwas(4.58±0.43)％、(23.52±1.87)％、(10.86±0.87)％,respectively.The SOD activities were(235.43±5.94)、(115.38±3.79)、(162.39±3.92)U·mg-1,respectively.MDA content were (1.27±0.28),(3.64±0.53),(2.33±0.57) nmol·mg-1.Scr were (63.26±5.21),(287.64±10.32),(106.47±6.29) μmol·L-1,respectively.BUN were (7.46±1.26),(13.65±1.04),(9.65±1.35) mmol·L-1.Compared with group A,the mRNA expression of Bax and caspase-3 were significantly increased,and the expression of Bcl-2 was decreased in group B (P<0.05).Compared with group B,the expression of Bcl-2 in was obvious increased,and the expression of Bax and caspase-3 decreased in group C(P<0.05).Conclusion GYY4137 reduces the level of oxidative stress and inhibit the apoptosis,Thus,it plays a protective role in the process of renal ischemia-reperfusion injury. |
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