| 田翰林,常柏,田文静.血糖波动对人脐静脉血管内皮细胞腺苷酸活化蛋白激酶和过氧化物酶增殖物激活受体γ共激活因子1α的影响[J].安徽医药,2019,23(2):250-253. |
| 血糖波动对人脐静脉血管内皮细胞腺苷酸活化蛋白激酶和过氧化物酶增殖物激活受体γ共激活因子1α的影响 |
| Effects of blood glucose fluctuation on AMPK and PGC-1α in human umbilical vein endothelial cells |
| 投稿时间：2017-07-20 |
| DOI： |
| 中文关键词: 血糖波动 能量代谢 腺苷酸活化蛋白激酶 过氧化物酶增殖物激活受体γ共激活因子1α 人脐静脉血管内皮细胞 |
| 英文关键词: Blood glucose fluctuation Energy metabolism AMPK PGC-1α HUVEC |
| 基金项目:国家自然科学基金(81473622、81273914);铜仁市科技课题(2018)52号 ◇临床医学◇ 血糖波动对人脐静脉血管内皮细胞腺苷酸活化蛋白激酶和过氧化物酶增殖物激活受体γ共激活因子1α的影响田翰林1,常柏2,田文静3(1.铜仁市碧江区中医医院,贵州 铜仁 554300;2.国家卫生健康委员会激素与发育重点实验室、天津医科大学代谢病医院内分泌研究所,天津 300070；3.贵州健康职业学院,贵州 铜仁 554300) |
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| 中文摘要: |
| 目的 观察血糖波动对人脐静脉血管内皮细胞(HUVEC) 腺苷酸活化蛋白激酶(AMPK)和过氧化物酶增殖物激活受体γ共激活因子1α(PGC-1α)的影响以探讨血糖波动对血管内皮损伤的机制。方法 体外培养HUVEC至第3代,将细胞分为:正常组:用5 mmol/L含葡萄糖和氨基酸的培养液(Glu DMEM)(模拟正常血糖环境)；高糖组:25 mmol/L Glu DMEM培养液(模拟高糖环境)；血糖波动组:交替用25 mmol/L和5 mmol/L Glu DMEM培养液,每8 h更换一次(模拟血糖波动环境),三组均培养48 h。使用流式细胞仪检测细胞凋亡率。蛋白质印迹法(Western blot)、反转录-聚合酶链反应(RT-PCR)检测各组AMPK和PGC-1α蛋白与mRNA表达。结果 高糖组与血糖波动组两组早/晚期细胞凋亡率高于正常组(P<0.001),且血糖波动组早/晚期细胞凋亡率高于高糖组(均P<0.05)；高糖组的AMPK和PGC-1α蛋白与mRNA表达分别为(0.232±0.018)、( 0.401±0.013),血糖波动组AMPK和PGC-1α蛋白与mRNA表达分别为(0.158±0.027) 、( 0.199±0.010),均比正常组的(0.905±0.032) 、( 0.946±0.045)降低(均P<0.05),且血糖波动组AMPK和PGC-1α蛋白与mRNA表达低于高糖组(均P<0.05)。结论 血糖波动对血管内皮细胞损伤较高糖状态更为严重,其机制可能与AMPK/PGC-1α相关通路及细胞能量代谢改变有关。 |
| 英文摘要: |
| Objective To observe the effect of blood glucose fluctuation on adenosine monophosphate activated protein kinase (AMPK) and peroxisome proliferators activated receptor gamma co-activator 1 alpha (PGC-1α) in human umbilical vein endothelial cells (HUVECs),and to explore the mechanism of blood glucose fluctuation on vascular endothelial injury.Methods The HUVECs were cultured in vitro for 3rd generation,and the cells were assigned into 3 groups:normal control group:5 mmol/L glucose (Glu) DMEM culture medium (Glu DMEM) (simulating normal blood glucose)；high glucose group:25 mmol/L glu DMEM culture medium (simulating high blood glucose environment)；glucose fluctuation group:alternate broth with 25 mmol/L and 5 mmol/L Glu DMEM,replaced once every 8 hours (simulating glucose fluctuations environment),and the three groups were all cultured for 48 hours.Flow cytometry was used to test its apoptosis rate.AMPK and PGC-1α protein and mRNA expression in each group were detected by Western blot and RT-PCR.Results Early/late cell apoptosis rates in both high glucose group and glucose fluctuation group were higher than that in the normal control group (all P<0.001),and the early/late apoptosis rate in the blood glucose fluctuation group was higher than that in the high glucose group (all P<0.05).The expressions of AMPK and PGC-1α protein and mRNA in high glucose group were (0.232±0.018),(0.401±0.013),respectively.The expressions of AMPK and PGC-1α protein and mRNA in glucose fluctuation group were (0.158±0.027),(0.199±0.010),respectively,which were lower than those in the normal control group [(0.905±0.032),(0.946±0.045),respectively] (all P<0.05).The expressions of AMPK and PGC-1α protein and mRNA in glucose fluctuation group was lower than those in high glucose group (all P<0.05).Conclusions Blood glucose fluctuation has more serious effect on the injury of vascular endothelial cells than high glucose,whose mechanism may be related to AMPK/PGC-1α-related pathway and cellular energy metabolism changed by blood glucose fluctuation. |
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