| 孙辉,何胜利.基于HPLC波长切换法对养胃宁胶囊中6种成分的质量控制研究[J].安徽医药,2019,23(3):473-476. |
| 基于HPLC波长切换法对养胃宁胶囊中6种成分的质量控制研究 |
| Quality control for six components in Yangweining capsule based on HPLC wavelength switching method |
| 投稿时间：2017-11-28 |
| DOI： |
| 中文关键词: 该方法简便、准确、重复性好,可为养胃宁胶囊多指标质量评价提供依据。 关键词:养胃宁胶囊 波长切换法 花旗松素 山姜素 乔松素 小豆蔻明 桤木酮 α-香附酮 |
| 英文关键词: Yangweining capsule Wavelength switching method Taxifolin Alpinetin Pinocembrin Cardamonin Alnustone α-cyperone |
| 基金项目: |
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| 摘要点击次数: 3001 |
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| 中文摘要: |
| 目的 建立同时测定养胃宁胶囊中花旗松素、山姜素、乔松素、小豆蔻明、桤木酮和α-香附酮含量的方法。 方法 采用Agilent Eclipse XDB C18(250 mm×4.6 mm,5 μm)；流动相:甲醇-0.1%磷酸溶液,梯度洗脱；流速:0.9 mL/min；柱温25 ℃。 结果 花旗松素、山姜素、乔松素、小豆蔻明、桤木酮和α-香附酮分别在2.18～54.50 μg/mL(r=0.999 9)、7.89～197.25 μg/mL(r=0.999 6)、6.57～164.25 μg/mL(r=0.999 8)、4.89～122.25 μg/mL(r=0.999 7)、5.76～144.00 μg/mL(r=0.999 9)、1.79～44.75 μg/mL(r=0.999 1)范围内线性关系良好,平均加样回收率分别为97.33%、100.18%、98.47%、97.42%、99.04%和96.99%,RSD分别为1.13%、0.59%、1.49%、1.36%、0.80%和0.95%。 |
| 英文摘要: |
| Objective To develop an HPLC wavelength switching method for determination of taxifolin,alpinetin,pinocembrin,cardamonin,alnustone and α-cyperone in Yangweining capsule simultaneously. Method The Agilent Eclipse XDB C18(250 mm×4.6 mm,5 μm) chromatographic column was adopted.The mobile phase was methanol-0.1% phosphoric acid solution with gradient elution,at a flow rate of 0.9 mL/min.The column temperature was set at 25 ℃. Results The linear response range were 2.18-54.50 μg/mL(r=0.999 9),7.89-197.25 μg/mL(r=0.999 6),6.57-164.25 μg/mL(r=0.999 8),4.89-122.25 μg/mL(r=0.9997),5.76-144.00 μg/mL(r=0.999 9),1.79-44.75 μg/mL(r=0.999 1) of taxifolin,alpinetin,pinocembrin,cardamonin,alnustone and α-cyperone,respectively.The average recoveries and the corresponding RSD were 97.33% (RSD＝1.13%),100.18% (RSD＝0.59%),98.47% (RSD＝1.49%),97.42% (RSD＝1.36%),99.04% (RSD＝0.80%),96.99% (RSD＝0.95%),respectively. Conclusion The method with good reproducibility is simple and accurate,which provide a new mind for quality assess of Yangweining capsule. |
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