| 陶春梅,龚雅利.铜绿假单胞菌280株基因分型及耐药性分析[J].安徽医药,2019,23(5):1032-1035. |
| 铜绿假单胞菌280株基因分型及耐药性分析 |
| Genotyping and drug resistance of 280 strains of clinically isolated pseudomonas aeruginosa |
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| DOI：10.3969/j.issn.1009-6469.2019.05.053 |
| 中文关键词: 铜绿假单胞菌 重复序列,核酸 肠杆菌属 聚合酶链反应 β内酰胺抗药性 基因,MDR |
| 英文关键词: Pseudomonas aeruginosa Repetitive sequences,nucleic acid Enterobacter Polymerase chain reaction Beta-lactam resistance Genes,MDR |
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| 中文摘要: |
| 目的 分析临床感染铜绿假单胞菌的基因型别、耐药特点以及对碳青霉烯类抗生素的耐药机制。方法 收集重庆市沙坪坝区陈家桥医院2011年6月至2017年7月共6年分离的280株铜绿假单胞菌,主要来源于呼吸内科和ICU病房的痰液标本,进行ERIC-PCR(肠杆菌基因间保守重复序列聚合酶链式反应)分型及药敏试验分析,并检测12种主要的碳青霉烯酶基因的携带情况。结果 分型结果表明主要存在A、G、H三种优势型别的流行,细菌对抗生素的耐药率普遍低于全国耐药监测数据,对碳青霉烯类抗生素亚胺培南和美罗培南的平均不敏感率分别为10.4%和7.1%。在35株碳青霉烯类抗生素不敏感菌株中,碳青霉烯酶IMP和VIM的携带率分别为8.57%和5.71%。结论 肺部感染以及A、G、H三种优势型别细菌是铜绿假单胞感染防控的重点对象,耐碳青霉烯类抗生素菌株中主要携带IMP和VIM两种常见的碳青霉烯酶基因,但阳性率较低。但碳青霉烯类抗生素耐药率逐年升高,仍应引起重视。 |
| 英文摘要: |
| Objective To investigate the genotype,drug resistance characteristics and mechanism of carbapenems resistance of Pseudomonas aeruginosa.Methods A total of 280 strains of Pseudomonas aeruginosa isolates from Chen Jiaqiao Hospital during June 2011 to July 2017 were analyzed by ERIC-PCR and antimicrobial susceptibility testing.The gene encoding carbapenemases was detected by PCR.Results A majority of the P.aeruginosa strains were isolated from sputum of the patients in medical and ICU wards.Type A,G,and H were the dominant types.Drug resistance rates in our hospital were significantly lower than that reported by China Antimicrobial Resistance Surveillance System.The average insensitivity rates to Imipenem and Meropenem were 10.4% and 7.1%,respectively.Only 8.57% and 5.71% of the carbapenem-non-susceptible isolates were positive for Carbapenemases IMP and VIM,respectively.Conclusion Pulmonary infection and type A,G,and H P.aeruginosa are the key points to control P.aeruginosa infection in our hospital.IMP and VIM were the main Carbapenemases harbored in the Carbapenem-non-susceptible isolates but with relatively low detection rate.It is still worthy of attention that resistance to Carbapenems is rising year by year. |
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