| 王昊,赵亮,王东升.肺癌耐顺铂细胞A549/CIS产生肿瘤干细胞特性的分子机制研究[J].安徽医药,2019,23(7):1306-1309. |
| 肺癌耐顺铂细胞A549/CIS产生肿瘤干细胞特性的分子机制研究 |
| Molecular mechanism of acquisition of cancer stem cell like characteristic in human cisplatin-resistant lung cancer cell line A549/CIS |
| 投稿时间：2018-03-31 |
| DOI： |
| 中文关键词: 肺癌 肿瘤干细胞特性 抗药性,肿瘤 磷脂酰肌醇3-激酶/丝氨酸-苏氨酸激酶 |
| 英文关键词: Lung cancer Cancer stem cell Drug resistance,neoplasm Phosphatidylinositol 3′-OH kinase(PI3K)/Protein kinase B |
| 基金项目:国家自然科学基金（81502599）；安徽省自然科学基金（1608085QH217） |
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| 中文摘要: |
| 目的 研究人肺癌耐顺铂细胞A549/CIS获得肿瘤干细胞特性的分子机制。方法 微球形成实验比较A549和A549/CIS细胞肿瘤干细胞特性的差别,以及磷脂酰肌醇3-激酶/丝氨酸-苏氨酸激酶(PI3K/AKT)通路抑制剂LY294002对A549/CIS细胞微球形成能力的影响。Western blotting检测A549和A549/CIS细胞中AKT、p65以及MAPK-p38信号通路的活化情况。CCK8实验检测A549和A549/CIS细胞对顺铂的敏感性,以及PI3K/AKT通路抑制剂LY294002对A549/CIS细胞对顺铂敏感性的影响。结果 A549/CIS细胞对顺铂的敏感性以及微球形成能力相对于A549明显增强,Western blotting结果表明:A549/CIS细胞中AKT通路明显被激活,而p65和MAPK-p38通路没有明显变化。LY294002可以抑制A549/CIS细胞的肿瘤干细胞特性,并提高A549/CIS细胞对顺铂的敏感性。结论 相对于敏感株细胞A549,耐药株A549/CIS细胞的肿瘤干细胞特性明显增强,而其机制涉及PI3K/AKT信号通路的激活。 |
| 英文摘要: |
| Objective To investigate the molecular mechanism of acquisition of cancer stem cell feature of cisplatin resistant human lung cancer cell line A549/CIS.Methods The capability of cancer stem cell feature of A549 cells and A549/CIS cells and the effect of LY294002 on cancer stem cell feature of A549/CIS cells were detected by mammosphere formation assay.Western blotting was used to detect the activation of AKT,p65 and MAPK-p38 signaling pathway.The sensibility of A549 cells and A549/CIS to cisplatin and the effect of LY294002 on sensibility of A549/CIS to cisplatin was measured by CCK8 assay.Results Compared to A549 cells,the resistance of A549/CIS cells to cisplatin and the cancer stem cell feature were significantly enhanced.The results of western blotting demonstrated that AKT signaling pathway is activated,not p65 and MAPK-p38 signaling pathways.LY294002 suppressed cancer stem cell feature of A549/CIS cells and enhanced the sensibility of A549/CIS cells to cisplatin.Conclusion Acquisition of cancer stem cell phenotype of human lung cancer cell line A549/CIS is linked with activation of PI3K/AKT signaling pathway. |
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