| 雷星,陈熹,赵琳,等.不同区域氧浓度在结肠癌细胞上皮间质转换-间质上皮转换过程中的作用[J].安徽医药,2019,23(9):1765-1768. |
| 不同区域氧浓度在结肠癌细胞上皮间质转换-间质上皮转换过程中的作用 |
| Epithelial to mesenchymal transition－mesenchymal to epithelial transition in colon cancer cells mediated by oxygen concentration in different regions |
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| DOI：10.3969/j.issn.1009-6469.2019.09.017 |
| 中文关键词: 上皮间质转换 间质上皮转换 细胞低氧 结肠癌 钙黏附蛋白E 波形蛋白 |
| 英文关键词: Epithelial to mesenchymal transition Mesenchymal to epithelial transition Cell hypoxia Colon cancer E-cadherin Vimentin |
| 基金项目:陕西省教育厅基金资助项目(15JK1818) |
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| 中文摘要: |
| 目的 探讨原发灶(低氧)及归巢部位(常氧或轻度高氧)氧浓度差异在上皮间质转换(EMT)-间质上皮转换(MET)过程的作用。方法 首先用差异氧浓度培养肿瘤细胞，以光镜，激光共聚焦显微镜观察肿瘤细胞形态变化。Transwell小室和划痕实验分别检测结肠癌细胞侵袭能力及迁移能力。RT-PCR检测钙黏附蛋白E(E-cadherin)、波形蛋白(Vimentin)表达量情况。结果 低氧环境下结COLO 205细胞较常氧及轻度高氧组明显发生EMT转换，降低E-cadherin表达，增强Vimentin表达；并相应伴随迁移、侵袭能力升高。结论 原发灶(低氧)及归巢部位(常氧或轻度高氧)的氧浓度差异是介导EMT-MET过程的重要因素。 |
| 英文摘要: |
| Objective The mechanisms of differences in oxygen concentrations of primary sites (hypoxic) and homing sites (normoxic or hyperoxic) by which primary sites (hypoxic) and homing sites (normoxic or hyperoxic) participate in epithelial to mesenchymal transition (EMT) and mesenchymal to epithelial transition (MET) conversion were evaluated.Methods Colon cancer cells were grown under different oxygen conditions，and cell morphology were examined by laser confocal microscopy and light microscopy.Transwell chambers were used to examine tumor invasiveness，and scratch assays were performed to examine cell migration.Reverse transcription-PCR was used to quantitate mRNA expression of E-cadherin，vimentin.Results Under hypoxic conditions，COLO 205 cells showed increased partial EMT，reduced E-cadherin expression，and increased vimentin expression，increased migration and invasiveness.Conclusion Differences in oxygen concentrations of primary sites (hypoxic) and homing sites (normoxic or hyperoxic) are important in the EMT-MET process. |
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