| 张博,刘阳,樊海鑫,等.优化ATP荧光法检测医院次氯酸钠消毒后物体表面菌落数的稳定性观察[J].安徽医药,2019,23(9):1830-1833. |
| 优化ATP荧光法检测医院次氯酸钠消毒后物体表面菌落数的稳定性观察 |
| Application of optimized ATP fluorescence method in NaClO disinfection |
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| DOI：10.3969/j.issn.1009-6469.2019.09.0034 |
| 中文关键词: 消毒检测 次氯酸钠 ATP荧光法 菌落总数 |
| 英文关键词: Disinfection test Sodium hypochlorite ATP fluorescence method Total number of colony |
| 基金项目:国家自然科学基金(31860315) |
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| 中文摘要: |
| 目的 观察优化ATP荧光法检测医院次氯酸钠消毒后物体表面菌落数的稳定性，拓展ATP荧光法在医院的应用范围。方法 优化ATP荧光法检测试剂的成分，采用优化后的ATP荧光法对医院一种常用消毒药次氯酸钠(NaClO)消毒后的菌落数进行检测，并考察其检测结果与国标平皿法菌落数检测结果的相关性。结果 优化后的ATP荧光法检测医院次氯酸钠消毒后的物体表面菌落总数与国标平皿法菌落总数检测结果有很好的相关性，相关系数为0.985，Y＝0.062 7X+12.453(R2＝0.962 5)，并在此基础上制定了荧光法检测的半定量标准。结论 ATP荧光法可以应用于医院次氯酸钠消毒后物表的检测。 |
| 英文摘要: |
| Objective This paper aims to by observing the number of colony after ATP fluorescence detectionto optimize andexpand the application scope of ATP fluorescence method in hospitals.Methods The composition of ATP fluorescence detection reagent was optimized，and then NaClO，a common disinfection component，was selected for determination.Results It was found that the optimized ATP fluorescence method whichused to detecthospital NaClO disinfection surface had a good correlation with the national standard plate method colony count detection results，Y＝0.062 7X+12.453 (R2＝0.962 5)，and on this basis，a semi-quantitative standard for fluorescence detection was established.Conclusion ATP fluorescence detection results have significant correlation with the national standard colony count detection method，with a correlation coefficient of 0.985.This method can be applied to the detection of the surface which disinfected after NaClO in hospitals. |
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