| 张付民,李艳兵.酸枣仁汤对睡眠剥夺大鼠视交叉上核生物钟基因 Period 1及 Period 2表达的影响[J].安徽医药,2019,23(11):2132-2136. |
| 酸枣仁汤对睡眠剥夺大鼠视交叉上核生物钟基因 Period 1及 Period 2表达的影响 |
| Effect of Suanzaoren Decoction on the expression of circadian clock genes Period 1 and Period 2 in the suprachiasmatic nucleus of rats with sleep deprivation |
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| DOI：10.3969/j.issn.1009?6469.2019.11.004 |
| 中文关键词: 酸枣仁汤 睡眠剥夺 视交叉上核 生物钟基因 Per1 Per2 机制 |
| 英文关键词: Suanzaoren Decoction Sleep deprivation Suprachiasmatic nucleus Circadian clock genes Per1 Per2 |
| 基金项目:国家自然科学基金项目( 81703973)；湖北省教育厅科研项目( Q20172003) |
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| 中文摘要: |
| 目的观察酸枣仁汤对睡眠剥夺大鼠视交叉上核( Suprachiasmatic nucleus，SCN)生物钟基因 Period 1(Per1)和 Period 2(Per2)表达的影响，并探讨酸枣仁汤改善睡眠的可能机制。方法将实验大鼠采用随机数字表法分为空白组、模型组、褪黑素组和酸枣仁汤组，除空白组外，其他各组腹腔注射对氯苯丙氨酸( PCPA)以制定睡眠剥夺模型，褪黑素组给予褪黑素溶液(36 mg·kg－1·d－1，10 mL·kg－1·d－1)，酸枣仁汤组给予酸枣仁汤水煎液( 4.86 g·kg－1·d－1，10 mL·kg－1·d－1)，空白组和模型组给予等体积的 0.9%氯化钠溶液( 10 mL·kg－1·d－1)连续灌胃 2周， 1次/天。末次给药后 24 h，用自主活动测试仪记录大鼠自主活动时间， Real time?PCR、免疫组化和 Western blot检，测 SCN中 Per1和 Per2的基因和蛋白表达水平。结果自主活动时间检测结果:空白组活动时间为( 377.79±39.55)s，模型组为( 478.43±38.89)s，褪黑素组为( 394.09±31.27)s，酸枣仁汤组为( 412.40± 36.24)s，空白组大鼠活动时间较少，造模后，与空白组比较，模型组大鼠活动时间明显增加( P＜0.01)；给药后，褪黑素组和酸枣仁汤组大鼠活动时间与模型组比较，都有不同程度减少( P＜0.01，P＜0.05)。 Real time?PCR和 Western blot检测结果:空白组大鼠 SCN中 Per1和 Per2的基因和蛋白表达水平较高，造模后，模型组大鼠 SCN中 Per1和 Per2的基因蛋白表达水平下降( P＜ 0.01)给药后，褪黑素组和酸枣仁汤组大鼠 SCN中 Per1和 Per2的基因和蛋白表达上升( P＜0.01，P＜0.05)。免疫组化检测结果:空白，组大鼠 SCN中 Per1和 Per2表达水平较高，造模后，模型组大鼠 SCN中 Per1和 Per2表达水平下降( P＜0.01)，给药后，褪黑素组大鼠 SCN中 Per1和 Per2表达上升( P＜0.01，P＜0.05)酸枣仁汤组大鼠 SCN中 Per1表达也上升( P＜0.05)Per2表达上升但差异无统计学意义( P＞0.05)。结论酸枣仁汤可以通过，调节大鼠 SCN中 Per1和 Per2的表达进而改善睡眠剥，夺大鼠 |
| 英文摘要: |
| Objective To observe the effect of Suanzaoren Decoction on the expression of Period 1(Per1)and Period 2(Per2)in the hypothalamic suprachiasmatic nucleus(SCN)of rats with sleep deprivation，and to explore the possible mechanism of Suanzaoren De? coction to improve sleep.Methods The experimental rats were randomly divided into blank group，model group，melatonin group and Suanzaoren Decoction group.Apart from blank group，PCPA was injected intraperitoneally into other groups to establish sleepdeprivation model.Melatonin group was given melatonin solution(36 mg·kg－1·d－1，10 mL·kg－1·d－1)and Suanzaoren Decoction group was given Suanzaoren Decoction(4.86g·kg－1·d－1，10 mL·kg－1·d－1)，the blank group and the model group were given the same volume of physiological saline(10 mL·kg－1·d-1)，continuous gastric perfusion for 2 weeks，once a day.After the last adminis? tration，the autonomic activity tester.Was used to record the spontaneous activity time of rats.The expression of Per1 and Per2 inSCN was detected by Real time?PCR，Immunohistochemistry and Western blot.Results Test results of independent activity time: The activity time of the rats in the blank group was less(377.79 ±39.55)s.After the model establishment，the activity time of the rats increased significantly［(377.79±39.55)s vs.(478.43±38.89)s］(P＜0.01).After the administration，the activity time of the rats in the melatonin group［(394.09±31.27)s］and the Suanzaoren group［(412.40±36.24)s］decreased to different degrees(P＜0.01， P＜0.05).rt?PCR and Western blot showed that the levels of Per1 and Per2 mRNA and protein expression in SCN were higher in blank group than in control group，which decreased(P＜0.01) after modeling.After administration，the levels of Per1 and Per2 mRNA and protein expression in SCN of melatonin group and Suanzaoren Decoction group increased(P＜0.01，P＜0.05).Immuno? histochemical results:The expression levels of Per1 and Per2 in SCN of rats in the blank group were higher，which were decreased after modeling(P＜0.01).After administration，Per1 and SCN in rats in melatonin group were administered.Per2 expression in? creased(P＜0.01，P＜0.05)，Per1 expression in SCN of Suanzaoren Decoction group also increased(P＜0.05)，Per2 expression in? creased，but with nothe no statistically significant difference(P＞0.05).Conclusion Suanzaoren Decoction can improve the sleep state of sleep deprived rats by regulating the expression of Per1 and Per2 in SCN of rat. |
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