| 马健钰.分拣微管连接蛋白 17在重症肌无力中的表达及相关性分析[J].安徽医药,2019,23(11):2239-2242. |
| 分拣微管连接蛋白 17在重症肌无力中的表达及相关性分析 |
| Correlation analysis of SNX17 protein expression and pathogenesis of myasthenia gravis and analysis of its mechanism |
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| DOI：10.3969/j.issn.1009?6469.2019.11.031 |
| 中文关键词: 重症肌无力 微管连接蛋白 连接蛋白类 胸腺组织 |
| 英文关键词: Myasthenia gravis Nexin Connexins Thymus |
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| 中文摘要: |
| 目的研究分拣微管连接蛋白 17(SNX17)蛋白在重症肌无力( MG)中的表达情况，并分析其与重症肌无力发病的关系，探讨其临床意义。方法选取 2014年 10月至 2016年 12月南阳市第一人民医院确诊的重症肌无力病人( MG组) 80例，健康对象(对照组) 50例，采用免疫组化法检测组织中 SNX17蛋白的表达，采用逆转录 ?聚合酶链反应( RT?PCR)法检测组织中 SNX17 mRNA的水平；用蛋白质印迹法检测 SNX17蛋白表达水平，分析其与病人临床病理参数关系。结果 SNX17 mRNA及蛋白在重症肌无力病人中表达水平( 78.75%)明显高于对照组( 32.00%)(P＝0.000)； SNX17蛋白的表达与病人组织学分化程度、临床病理学分期及淋巴结转移情况无明显关系( P＝0.000)。结论 SNX17蛋白与重症肌无力发病具有密切的关联，与临床病理参数无明显的关系。 |
| 英文摘要: |
| Objective To investigate the expression of sorting microtubule associated protein 17(SNX17)protein in myasthenia gravis and analyze the relationship between SNX17 and the pathogenesis of myasthenia gravis，and the clinical significance of which were discussed.Methods Total of 80 patients with myasthenia gravis(group MG)and 50 healthy subjects(control group) accepted in our hospital between October 2014 and December 2016 were selected.Immunohistochemical method was used to detectthe expression of SNX17 protein in tissues.The the levels of SNX17 mRNA in tissues were detected by RT?PCR.The expression ofSNX17 protein was detected by western?blot.The relationship between the parameters and clinical pathological parameters were ana?lyzed.Results The expression levels of SNX17mRNA and protein of patients with myasthenia gravis were significantly higher thanthose of the control group(78.75% vs. 32.00%)( P＝0.000).The expression of SNX17 protein was not correlated with histological differentiation，clinicopathological staging and lymph node metastasis(P＝0.000).Conclusion SNX17 protein is closely related to the pathogenesis of myasthenia gravis，and has no obvious relationship with clinical pathological parameters. |
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