| 夏清荣,单锋,徐亚运,等.限制性片段长度多态性聚合酶链反应法检测人细胞色素氧化酶 2D6药物代谢相关基因位点多态性[J].安徽医药,2019,23(12):2347-2350. |
| 限制性片段长度多态性聚合酶链反应法检测人细胞色素氧化酶 2D6药物代谢相关基因位点多态性 |
| Detection of CYP2D6 drug metabolism related locus polymorphism by PCR?RFLP |
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| DOI：10.3969/j.issn.1009?6469.2019.12.004 |
| 中文关键词: 细胞色素 P450 CYP2D6 多态性，限制性片段长度 扩增片段长度多态性分析 基因检测 |
| 英文关键词: Cytochrome P?450 CYP2D6 Polymorphism,restriction fragment length Amplified fragment length polymorphism analysis Genetic testing |
| 基金项目: |
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| 摘要点击次数: 1979 |
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| 中文摘要: |
| 目的建立测定人细胞色素氧化酶 2D6(CYP2D6)基因多态性的限制性片段长度多态性聚合酶链反应(polymerase chain reaction?restriction fragment length polymorphism，PCR?RFLP)检测方法。方法通过增加 PCR扩增体系中 DNA模板上样体积、降低引物浓度以及减少限制性内切酶的用量等方法改进、优化实验条件，建立以 PCR?RFLP法为基础的肝药酶 CYP2D6基因型检测方法。结果采用 PCR?RFLP法能够检测 CYP2D6?2、CYP2D6?10和 CYP2D6?14基因型，且过程简便，经济实用。结论该方法检测 CYP2D6药物代谢相关基因位点基因型具有简便、高效、准确和经济的特点，可在临床及实验室推广使用。 |
| 英文摘要: |
| Objective To establish a method for the determination of human cytochrome P450 2D6(CYP2D6)gene polymorphism by polymerase chain reaction?restriction fragment length polymorphism(PCR?RFLP).Methods The experimental conditions were improved and optimized by increasing the sample size of DNA template，reducing the primer concentration and the amount of re?striction endonuclease in PCR amplification system.The method for detecting the CYP2D6 genotype of liver drug enzyme based onPCR?RFLP method was established.Results The CYP2D6?2，CYP2D6?10 and CYP2D6?14 genotypes could be detected by PCR?RFLP method，and the process was simple and economical.Conclusion PCR?RFLP method is simple，efficient，accurate and eco? nomical for detecting genotypes of CYP2D6 drug?related gene loci and can be used in clinical and laboratory applications. |
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