| 黄和军,崔小兵,杨军辉.一测多评法测定槐花中 3种黄酮类成分的含量[J].安徽医药,2019,23(12):2366-2370. |
| 一测多评法测定槐花中 3种黄酮类成分的含量 |
| Determination of three flavonoids ingredients of Sophorae Flos by QAMS method |
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| DOI：10.3969/j.issn.1009?6469.2019.12.008 |
| 中文关键词: 槐花 色谱法，高效液相 芦丁 山柰酚 ?3?O?芸香糖苷 槲皮素 一测多评(QAMS) |
| 英文关键词: Sophorae Flos Chromatography,high performance liquid chromatography Rutin Kaempferol?3?O?rutinoside Quercetin Quantitative analysis of multi?components by single?marker(QAMS) |
| 基金项目:国家科技重大专项(2009ZX09308?004) |
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| 摘要点击次数: 1916 |
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| 中文摘要: |
| 目的建立一测多评法(QAMS)用于测定槐花中芦丁，山柰酚?3?O?芸香糖苷及槲皮素 3种黄酮类成分的含量。方法芦丁作为内标物，计算芦丁与槲皮素和山柰酚 ?3?O?芸香糖苷的相对校正因子，分别采用 QAMS和外标法测定 40批槐花药材中以芦丁，山柰酚 ?3?O?芸香糖苷和槲皮素的含量，比较两种测定方法之间的差异。结果 QAMS与外标法所测得的槐花中芦丁、山柰酚 ?3?O?芸香糖苷和槲皮素的含量分别为(9.98±1.39)%、(10.08±1.38)%，差异无统计学意义(t＝0.324，P＝0.747)。结论 QAMS准确可行，可用于槐花中 3种黄酮类成分测定。 |
| 英文摘要: |
| Objective To establish a quantitative analysis of multi?components by single marker(QAMS)for the determination of three flavonoids ingredients in Sophorae Flos，i.e.rutin，kaempferol?3?O?rutinoside，quercetin.Methods Rutin was used as the ref? erence substance，the relative correction factors of kaempferol?3?O?rutinoside and quercetin were established，the contents of rutin， kaempferol?3?O?rutinoside and quercetin in 40 batches of Sophorae Flos were determined by QAMS and external standard method respectively，and the differences between the two methods were compared.Results The contents of rutin，aempferol?3?O?rutinoside and quercetin in Sophorae Flos measured by QAMS and external standard method were(9.98±1.39)% and(10.08±1.38)%，re? spectively，with no statistical significance(t＝0.324，P＝0.747).Conclusion It is feasible and accurate to determine the contents of three flavonoids components in Sophorae Flos by QAMS method. |
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