| 刘璐璐,唐敏,宋莎莎,等.十二指肠乳头区精细注射法诱导大鼠急性重症胰腺炎模型[J].安徽医药,2019,23(12):2374-2377. |
| 十二指肠乳头区精细注射法诱导大鼠急性重症胰腺炎模型 |
| Study on acute severe pancreatitis induced by fine injection of duodenal papilla region |
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| DOI：10.3969/j.issn.1009?6469.2019.12.010 |
| 中文关键词: 胰腺炎，急性坏死性 牛磺胆酸钠 疾病模型，动物 肝胰管壶腹 注射 大鼠， Sprague?Dawley 十二指肠乳头区精细注射法 |
| 英文关键词: Pancreatitis,acute necrotizing Taurocholic acid Disease models,animal Ampulla of vater Injections Rats,sprague?dawley Method of accurate nipple injection |
| 基金项目:安徽省自然科学基金(1708085QH192) |
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| 中文摘要: |
| 目的观察十二指肠乳头区精细注射法建立大鼠急性重症胰腺炎模型的效果。方法大鼠通过简单随机化分组的方法分为:假手术组、精细注射组(6h、24 h)、被膜下组(6h、24 h)，每组 15只。精细注射组大鼠采用十二指肠乳头朝向胆管开口的部位精细注射，被膜下组采用胰腺被膜下多点注射，均采用胰岛素笔注射，注射药物均为 5%牛磺胆酸钠(1 mL/100 g)。比较各组死亡率、手术时间、血清淀粉酶含量、胰腺组织病理组织学变化及成模率。结果(1)被膜下组有两只大鼠死亡，各组间死亡率比较差异无统计学意义(P＞0.05)。(2)被膜下组较精细注射组手术时间明显缩短［(12.80±1.15)min比(14.87±1.19) min，P＜0.05］。(3)与假手术组(233.13±67.75)U/L比较，精细注射组［ 6h:(2 535.07±999.83)U/L，24 h:(4 161.87±2 437.02)U/L］和被膜下组［ 6h:(3 401.54±870.05)U/L，24 h:(3 503.67±1 540.60)U/L］血清淀粉酶含量均显著升高(P＜0.05)。与精细注射组 6h比较，精细注射组 24 h的血清淀粉酶含量明显升高(P＜0.05)。(4)精细注射组 24 h可见广泛凝固性坏死伴出血，坏死区细胞结构不清，实质内大量炎症细胞浸润。与假手术组比较，被膜下组和精细注射组的胰腺炎病理评分均显著升高(P＜ 0.05)且精细注射组 24 h的评分升高最显著(P＜0.05)。(5)假手术组、被膜下组 6h和精细注射组 6h均无成模大鼠。精细注射组24，h成模率 100%(15/15)最高(P＜0.05)。结论十二指肠乳头区精细注射法建立大鼠急性重症胰腺炎模型具有血清淀粉酶含量变化显著、胰腺组织病理典型、成模率高的优点，可为胰腺炎基础和临床研究提供基础，值得推广。 |
| 英文摘要: |
| Objective To investigate the effect of fine injection of duodenal papilla area on the establishment of severe acute pan?creatitis models in rats.Methods The rats were randomly divided by simple randomization into:the sham operation group，the fine injection group(6h，24 h)，the subcapsular group(6h，24 h)，15 in each group.The rats in the fine injection group were in?jected at the site of the duodenal papilla facing the orifice of the bile duct，while the rats in the subcapsular group applied multiple points injection under pancreatic capsule，they were injected with 5% Bile Salts(1 mL/100 g).The mortality，operation time，serum amylase content，pathological changes and modeling rate of pancreas were compared.Results(1)Two rats died in the subcapsular group，but there was no statistical significance between the groups(P＞0.05)(.2)The operation time in the subcapsular group was significantly shorter than that in the fine injection grou［(12.80±1.15)min vs.(14.87±1.19)min，P＜0.05］(3)Compared with .the sham operation group(233.13±67.75)U/L，the hemodlastase contents in the fine injection group［6h:(2 535.07±999.83)U/L， 24 h:(4 161.87±2 437.02)U/L］and the subcapsular group［6h:(3 401.54±870.05)U/L，24 h:(3 503.67±1 540.60)U/L］were sig? nificantly increased(P＜0.05).Compared with the fine injection group at 6 h，the hemodlastase content in the fine injection group at 24 h was significantly increased(P＜0.05).extensive coagulation necrosis with hemorrhage was (4)In the fine injection group， observed at 24 h，cell structure in the necrotic area was unclear，and a large number of inflammatory cells were infiltrated in the pa?renchyma.Compared with the sham operation group，the pathological scores of pancreatitis of the subcapsular group and the fine in? jection group were significantly increased(P＜0.05)，and the score of the fine injection group at 24 h was the most significant in? crease(P＜0.05).the subcapsular group at 6 h and the fine injection(5)There were no model rats in the sham operation group， group at 6 h.The rate of model formation was the highest in the fine injection group at 24 h(P＜0.05).Conclusion The method of fine injection of duodenal papilla area has the advantages of remarkable changes in hemodlastase content，typical pathology ofpancreatic tissue and high rate of model formation.It can provide the basis for the basic and clinical study of pancreatitis，it is worth popularizing. |
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