| 杨林,贾钦尧,宋珊.香烟提取物对人支气管上皮细胞抗衰老因子 Klotho的影响分析及应对措施的效果研究[J].安徽医药,2019,23(12):2450-2453. |
| 香烟提取物对人支气管上皮细胞抗衰老因子 Klotho的影响分析及应对措施的效果研究 |
| Analysis of the effect of cigarette extract on Klotho factor in HBE cells |
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| DOI：10.3969/j.issn.1009?6469.2019.12.030 |
| 中文关键词: 上皮细胞 支气管 肺疾病，慢性阻塞性 白细胞介素类 肿瘤坏死因子 α 香烟提取物 人支气管上皮细胞 抗衰老因子 Klotho |
| 英文关键词: Epithelial cells Bronchi Pulmonary disease,chronic obstructive Interleukins Tumor necrosis factor?alpha Cigarette extract HBE cells Klotho factor |
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| 目的分析香烟提取物对人支气管上皮细胞(HBE)抗衰老因子 Klotho的影响及应对措施的效果。方法选择 2016年 3月至 2017年 8月在仪陇县人民医院进行体检的健康人员 30例作为对照组，选择同期在该院治疗的具有 10年吸烟史慢性阻塞性肺疾病(简称“慢阻肺”)病人 30例作为观察组，对两组人群外周血细胞因子白细胞介素(IL)?8、IL?6、肿瘤坏死因子(TNF)?α、 Klotho蛋白表达水平进行评估。同时以香烟提取物刺激 HBE细胞构建支气管炎症模型，并采用地塞米松进行干预。结果慢阻肺病人外周血 IL?8，IL?6及 TNF?α含量均显著高于对照组，差异有统计学意义(t＝3.012，P＝0.013；t＝4.092，P＝0.000；t＝ |
| 英文摘要: |
| objective to analyze the effect of cigarette extract on Klotho factor in HBE cells.Methods from March 2016 to August 2017，20 healthy persons in our hospital were selected as control group and 30 patients with chronic obstructive pulmonary disease(COPD)with 10 years of smoking history treated in our hospital as observation group.The expression of IL?8，IL?6，TNF?α and Klotho protein in peripheral blood was evaluated.At the same time，bronchitis model was established with cigarette extract to stimu? late bronchial epithelial cells and treated with dexamethasone.Results The content of IL?8，IL?6 and TNF?α in patients with chronic obstructive pulmonary disease were significantly lower than those in the control group(t＝3.012，P＝0.013；t＝4.092，P＝ 0.000；t＝3.421，P＝0.008)，and the content of Klotho protein in peripheral blood of patients with chronic obstructive pulmonarydisease was significantly lower than that of the control group［(0.000 23±0.000 03)pg/mL vs.(0.008 42±0.000 01)pg/mL，t＝ 4.112，P＝0.000］.There was a significant difference(P＜0.05)between group B and group B(P＜0.05).The content of IL?8，IL? 6，TNF?α in group B was significantly higher than that in group A(t＝2.917，P＝0.025；t＝3.411，P＝0.000；t＝3.498，P＝0.000). The expression level of the mRNA of IL?8，IL?6 and TNF?α in group B was higher than that in group A(t＝2.802，P＝0.027；t＝ 3.212，P＝0.000；t＝3.092，P＝0.000).Conclusion Cigarette extract can significantly reduce the content of Klotho factor and up?regulate the expression of gene of many inflammatory factors in HBE cells in vitro.Dexamethasone treatment in vitro can reducethe inflammatory response of HBE cells. |
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