| 管群,张秦,任青玲.子宫内膜癌中癌症相关区域长链非编码 RNA5表达意义及预后价值[J].安徽医药,2019,23(12):2480-2483. |
| 子宫内膜癌中癌症相关区域长链非编码 RNA5表达意义及预后价值 |
| Expression of cancer?associated region long non?coding RNA 5 in endometrial carcinoma and its value in predicting the prognosis |
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| DOI：10.3969/j.issn.1009?6469.2019.12.037 |
| 中文关键词: 子宫内膜肿瘤 长链非编码 RNA 肿瘤标记，生物学 实时聚合酶链反应 临床病理 预后 |
| 英文关键词: Endometrial neoplasms Long non?coding RNA Tumor markers,biological Real?time polymerase chain reaction Clinical pathology Prognosis |
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| 中文摘要: |
| 目的探讨子宫内膜癌中癌症相关区域长链非编码 RNA5(CARLo?5)表达意义及对预后的预测价值。方法于 2011年 1月至 2013年 1月，采用实时荧光定量多聚核苷酸链式反应(qPCR)检测采集于南京中医药大学附属医院的 79份子宫内膜癌组织标本和 50份正常子宫内膜组织标本 CARLo?5表达水平，并分析其与子宫内膜癌病人临床病理参数及总生存期的关系。所有病人均接受至少 5年的随访，记录病人的 5年总生存期(OS)和无进展生存期(PFS)。结果子宫内膜癌组织 CARLo?5表达量(3.76±0.78)显著高于正常子宫内膜组织(0.38±0.12)，差异有统计学意义(P＜0.01)； CARLo?5表达量与年龄、子宫内膜癌病理类型无关(P＞0.05)与国际妇产科联盟(FIGO)分期、细胞分化程度、血管浸润程度、淋巴结转移情况有关(P＜0.05)；截止随访时间， 5年 PFS率为50，.63%(40/79)5年 OS率为 68.35%(54/79)；无病生存者 CARLo?5表达量低于带瘤生存者，存活者 CARLo?5表达量低于死亡者，差异有统计学，意义(P＜0.01)； Cox多因素分析显示，年龄、 FIGO分期、细胞分化程度、血管浸润程度、淋巴结转移、 CARLo?5表达与子宫内膜癌病人 OS有关(P＜0.05)。经受试者工作特征曲线(ROC)的绘制和分析， CARLo?5表达量的临界值为 3.31时，预测 5年生存率的灵敏度和特异度最高， AUC为 0.817，灵敏度和特异度分别为 76.0%、77.8%；3.31为 CARLo?5的临界值，分为 CARLo?5≤3.31组(51例)和 CARLo?5＞3.31组(28例)两组 5年 OS率分别为 78.43%(40/51)、以50.0%(14/28)，两组比较差异有统计学意义(P＜0.05)。结论 CARLo?5高表达可能子宫内膜癌发生、发展，且其是影响参与，病人预后的独立危险因素，可用于预测子宫内膜癌病人预后。 |
| 英文摘要: |
| Objective To investigate the significance of the expression of cancer?associated region long non?coding RNA(CARLo? 5)in endometrial carcinoma and its value in predicting the prognosis.Methods The expression levels of CARLo?5 in 79 speci?mens of endometrial carcinoma and 50 normal endometrial tissue specimens which were collected from the Affiliated Hospital ofNanjing University of Traditional Chinese Medicine between January 2011 and January 2013 were detected by real?time fluorescentquantitative polymerase chain reaction(qPCR).The relationship between clinicopathological parameters and the overall survival ofpatients with endometrial carcinoma was analyzed.All patients were followed for at least 5 years，and the 5?year overall survival(OS)and progression?free survival(PFS)were recorded.Results The expression level of CARLo?5 in endometrial carcinoma tis?sues was significantly higher than that in normal endometrial tissues［(3.76±0.78)vs.(0.38±0.12)］(P＜0.01).The expression of CARLo?5 was not related to age o the pathological type of endometrial carcinoma(P＞0.05)，but it was related to the International Federation of Gynecology and Obstetrics(FIGO)staging，cell differentiation degree，degree of vascular invasion or lymph node me? tastasis(P＜0.05).At the end of follow?up，the 5?year PFS rate and OS rate were 50.63%(40/79)and 68.35%(54/79)，respective? ly.The expression quantity of CARLo?5 in disease?free survivors was lower than that in tumor?survivors，and it was lower in survi? vors than in the dead(P＜0.01).Cox multivariate analysis showed that age，FIGO staging，cell differentiation degree，degree of vas? cular invasion，lymph node metastasis and the expression of CARLo?5 expression were related to the OS of patients with endometri? al carcinoma(P＜0.05).The receiver operating characteristic(ROC)curve analysis showed that when the critical value of the ex? pression quantity of CARLo?5 was 3.31，the sensitivity and specificity in predicting the 5?year survival rate were the highest.The AUC，sensitivity and specificity were 0.817，76.0% and 77.8%，respectively.With 3.31 as the critical value，the patients were divid? ed into ≤ 3.31 group(51 cases)and＞3.31 group(28 cases).The 5?year OS rates of the two groups were 78.43%(40/51)and 50.0%(14/28)respectively(P＜0.05).Conclusion The high expression of CARLo?5 may be involved in the occurrence and de? velopment of endometrial carcinoma，and it is an independent risk factor for the prognosis.It can be used for predicting the progno?sis of patients with endometrial carcinoma. |
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