| 严必红,曾为红.Bcl2相互作用蛋白 3在子宫内膜组织中的表达及其在子宫内膜异位症发病中的意义[J].安徽医药,2020,24(1):136-140. |
| Bcl2相互作用蛋白 3在子宫内膜组织中的表达及其在子宫内膜异位症发病中的意义 |
| The significance of BNIP3 in the pathogenesis of endometriosis analyzing through the methods of molecular biology and bioinformatics |
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| DOI：10.3969/j.issn.1009?6469.2020.01.034 |
| 中文关键词: 凋亡调节蛋白质类 子宫内膜异位症 原癌基因蛋白质 c?bcl?2 生物信息学 Bcl2/腺病毒 E1B相互作用蛋白 3 凋亡 |
| 英文关键词: Apoptosis regulatory proteins Endometriosis Proto?oncogene proteins c?bcl?2 Bioinformatics Bcl2/Adenovirus E1B 19kDa interacting protein 3 Apoptosis |
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| 中文摘要: |
| 目的探究 Bcl2相互作用蛋白 3(BCL2 Interacting Protein 3，BNIP3)表达变化与正常子宫内膜周期的关系及在子宫内膜异位症( EMs)发病中的意义。方法从 GEO数据库获得 GSE51981、GSE6364、GSE4888、GSE25628的基因表达谱，通过生物信息学手段比较正常子宫内膜周期组增殖期到分泌期基因表达变化，分析基因富集情况以及 BNIP3在正常子宫内膜月经周期中的表达变化；比较数据集中正常子宫内膜周期组人群与内异症病人差异趋势表达基因以及各样本中 BNIP3的表达，总结其在 EMs发病中的意义；取 23例Ⅲ?Ⅳ EMs病人正位及异位子宫内膜组织，以及 25例因子宫肌瘤行子宫切除术病人的子宫内膜组织作为正常对照，通过实时荧光定量 qRT?PCR及蛋白质印迹法( Western Blot)检测 BNIP3在各组织中的表达，验证 BNIP3表达变化同 EMs发病的关系。结果 GSE4888、GSE6364、GSE51981数据集中， BNIP3在增殖期与分泌期相对表达量分别为 100.22±16.54、332.80±32.29，487.68±100.09、1425.83±157.47，8.36±0.69、9.82±0.71，均差异有统计学意义( P＜0.0001)； GSE25628数据集中， BNIP3在正常子宫内膜组织、 EMs病人正位、 EMs病人异位子宫内膜组织中的表达量分别为( 10.11± 0.72)、(9.19±0.56)、(8.52±0.57)三组间对比差异有统计学意义；临床样本中，正常内膜组织、 EMs正位内膜组织、 EMs异位内膜组织中 BNIP3的 mRNA表达别为 1.15±0.57、0.56±0.19、0.36±0.08，三组间对比差异有统计学意义； BNIP3蛋白在正常子量分，宫内膜组织、 EMs正位内膜组织、 EMs异位内膜组织中的表达水平分别为 1.00±0.41、0.58±0.20、0.38±0.12，三组间对比均差异有统计学意义。结论 BNIP3在子宫内膜组织中的表达降低与 EMs的发生具有相关性。 |
| 英文摘要: |
| Objective To explore the relationship between BNIP3 expression and normal endometrial cycle and its significance inthe pathogenesis of endometriosis(EMs).Methods Gene expression profiles of GSE51981，GSE6364，GSE4888 and GSE25628 were obtained from the GEO database.First，the changes of gene expression of normal endometrial tissues in the proliferative phaseand the secretory phase were compared by bioinformatics method，and the gene enrichment and BNIP3 expression were analyzed. Secondly，the differential expressed genes of normal population and endometriosis patients in the data set and the expression ofBNIP3 in each sample were analyzed.Finally，the endometrial tissues of 23 patients with Ⅳ?Ⅳ EMs and 25 patients who underwenthysterectomy for uterine fibroids were taken as normal controls.The expression of BNIP3 in all tissues was detected by qRT?PCRand Western Blot to verify the relationship between BNIP3 expression and the incidence of EMs.Results In the data set of GSE4888，GSE6364 and GSE51981，the relative expressions of BNIP3 in the proliferation and secretion phases were(100.22± 16.54)vs.(332.80±32.29),(487.68±100.09)vs.(1425.83±157.47)vs.(8.36±0.69)vs.(9.82±0.71)respectively，and the differ? ences were statistically significant(P＜0.0001).According to GSE25628 data set，the expression of BNIP3 in in normal endometri? al tissues，EMs orthotopic endometrial tissues and EMs ectopic endometrial tissues were(10.11±0.72)，(9.19±0.56)，and(8.52± 0.57)，respectively.Among the clinical samples，the mRNA expressions of BNIP3 in normal endometrial tissues，EMs orthotopic en? dometrial tissues and EMs ectopic endometrial tissues were 1.15±0.57，0.56±0.19 and 0.36±0.08，respectively.The expression levels of BNIP3 protein in normal endometrial tissues，EMs orthotopic endometrial tissues and EMs ectopic endometrial tissues were 1.00±0.41，0.58±0.20 and 0.38±0.12，respectively.Conclusion The change of BNIP3 expression in endometrial tissue is closely related to the maintenance of normal menstruation，and the decrease of BNIP3 expression is correlated with the occurrence of EMs. |
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