| 陈通,陈焕诗,易军飞.过氧化氢酶表达与腰椎管狭窄症病人黄韧带肥大[J].安徽医药,2020,24(4):681-684. |
| 过氧化氢酶表达与腰椎管狭窄症病人黄韧带肥大 |
| Study on the expression of catalase in ligamentum flavum hypertrophy |
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| DOI：10.3969/j.issn.1009?6469.2020.04.011 |
| 中文关键词: 椎管狭窄/病因学 过氧化氢酶 黄韧带 椎间盘移位 弹性蛋白 |
| 英文关键词: Spinal stenosis/etiology Catalase Ligamentum flavum Intervertebral disc displacement Elastin |
| 基金项目:广西壮族自治区卫生和计划生育委员会自筹经费科研课题( Z20170015) |
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| 中文摘要: |
| 目的研究过氧化氢酶基因在腰椎管狭窄症( LSCS)病人肥大黄韧带标本中的表达。方法黄韧带标本通过外科手术获自 2013年 1月至 2015年 12月间柳州市柳铁中心医院 75例椎管狭窄病人( LSCS组)和 75例腰椎间盘突出症病人( LDH组)。使用卡尺在最厚点测量黄韧带厚度至 0.01 mm的精度。黄韧带弹性蛋白降解和纤维化的程度分别通过苏木精和伊红染色和 Masson三色染色分级( 0～4级)。比较两组之间的黄韧带测量值，组织学数据和免疫组织学结果。结果 LSCS组的平均黄韧带厚度显著高于 LDH组(分别为 5.99 mm和 2.95 mm，P＝0.004)。脊柱狭窄样本中弹性蛋白降解和黄韧带纤维化明显比椎间盘突出样本严重［(3.04±0.50)比( 0.79±0.60)，P＝0.007；(3.01±0.47)比( 0.66±0.42)，P＝0.009)］。结论过氧化氢酶的表达降低与 LSCS黄韧带肥大有关。 |
| 英文摘要: |
| Objective To study the expression of catalase gene in the ligamentum flavum fatty(LF)of patients with lumbar spinal stenosis(LSCs).Methods The ligamentum flavum specimens were obtained from 75 patients with spinal stenosis(LSCs group) and 75 patients with lumbar disc herniation(LDH group).Measure the thickness of the ligamentum flavum to an accuracy of 0.01mm at the thickest point with a caliper.The degradation and fibrosis of elastin in ligamentum flavum were graded by hematoxylinand eosin staining and Masson trichrome staining(0?4 grade).The results of measurement，histology and immunohistochemistry were compared between the two groups.Results The average LF thickness was significantly higher in the LSCS group than in the LDH group(5.99 mm vs.2.95 mm，P＝0.004). Elastin degradation(3.04±0.50 vs.0.79±0.60，P＝0.007)and fibrosis(3.01±0.47 vs.0.66±0.42，P＝0.009)of the LF were significantly more severe in spinal stenosis samples than in the disc herniation samples. Conclusion The decreased expression of catalase is associated with LF hypertrophy in patients with LSCS. |
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