| 吴子静,梁大虎,杨斌,等.高效液相色谱-串联质谱法同时测定五味子糖浆中 7种木脂素的含量[J].安徽医药,2020,24(5):869-873. |
| 高效液相色谱-串联质谱法同时测定五味子糖浆中 7种木脂素的含量 |
| Simultaneous determination of seven lignan components in Wuweizi syrup by HPLC?MS/MS |
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| DOI：10.3969/j.issn.1009?6469.2020.05.005 |
| 中文关键词: 木脂素类 色谱法，高压液相 串联质谱法 五味子糖浆 液相 ?质谱联用 含量测定 |
| 英文关键词: Lignans Chromatography,high pressure liquid Tandem mass spectrometry Wuweizi syrup HPLC?MS/MS Content determination |
| 基金项目:皖南医学院中青年科研基金(WK2018F05) |
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| 中文摘要: |
| 目的建立一种高效液相色谱 -串联质谱( HPLC?MS/MS)同时测定五味子糖浆中 7种木脂素成分含量的方法。方法采用 Agilent ZORBAX Eclipse XDB?C18(4.6 mm×150 mm，5 μm)色谱柱，以 90%甲醇(含 0.1%甲酸) ?水(含 0.1%甲酸， 5 mmol乙酸铵)为流动相，流速 0.5 mL/min，柱温 30 ℃，进样量: 10 μL；采用电喷雾电离源( ESI)正离子扫描，多反应离子监测模式( MRM)进行定量分析。结果五味子糖浆中 7种木脂素成分分离度良好，在测定范围内线性，关系均良好( r＞0.995)，平均回收率分别为 98.66%，92.41%，95.15%，100.46%，96.67%，100.94%，98.83%。结论该方法快速、灵敏、可靠，可满足五味子糖浆中五味子醇甲、五味子醇乙、五味子甲素、五味子乙素、五味子丙素、五味子酚和五味子酯甲的含量测定。 |
| 英文摘要: |
| Objective To establish a method for simultaneous determination of seven lignan components in Wuweizi syrup by HPLC? MS/MS.Methods The chromatographic separation was performed on a Agilent ZORBAX Eclipse XDB?C18(4.6 mm×150 mm，5 μm)column with the mobile phase consisting 90% methanol(containing 0.1% formic acid)?water(containing 0.1% formic acid，5 mmol ammonium acetate)at a flow rate of 0.5 mL/min，the column temperature was 30 ℃ and the injection volume was 10 μL.An electrospray positive ion multiple reaction monitoring(MRM)was used as a detector.Results The seven lignan components in Wuweizi syrup had good separation and good linearity in the determination range(r＞0995).The average recoveries were 98.66%，92.41%，95.15%，100.46%，96.67%，100.94% and 98.83%，respectively.Conclusion This method is rapid，sensitive and reliable， which can be used to determine the content of schisandrin，schisandrol B，deoxyschizandrin，schizandrin B，schizandrin C，schisanhe?nol and schisantherin A in Wuweizi syrup. |
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