| 许妍妮,赵俐婷,贺芳,等.葡萄籽提取物对破骨细胞分化的影响[J].安徽医药,2020,24(8):1502-1504. |
| 葡萄籽提取物对破骨细胞分化的影响 |
| Effects of grape seed extract on osteoclast differentiation |
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| DOI：10.3969/j.issn.1009?6469.2020.08.004 |
| 中文关键词: 葡萄籽提取物 破骨细胞 组织蛋白酶 K RAW264.7细胞 抗氧化 |
| 英文关键词: Grape seed extract Osteoclasts Cathepsin K RAW264.7 cell Antioxidation |
| 基金项目:国家自然科学基金项目(81670806，81972133)；中央高校基本科研业务费专项资金(xzy012019094)；陕西省自然科学基础研究计划(2020JM-365) |
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| 中文摘要: |
| 目的检测葡萄籽提取物对破骨细胞分化、形成、功能的影响。方法利用实时定量基因扩增荧光检测系统(qPCR)检测破骨细胞分化标志分子的表达，利用抗酒石酸酸性磷酸酶(TRAP)染色及 TRAP活性定量实验检测破骨细胞的形成情况，利用c?TX定量实验检测破骨细胞的骨吸收活性。结果细胞克隆经诱导分化后，与对照组相比， GSE组细胞的破骨标志分子 Itgb3 mRNA相对表达量由(216.59±34.61)下降至(104.55±9.54)(n＝3，P＝0.0355)； Acp5由(103.37±14.77)下降至(78.96±13.15)(n＝3，P＝0.0351)； Dcst由(304.85±14.77)下降至(65.03±11.75)(n＝3，P＝0.0003)； Ctsk由(290.23±41.65)下降(132.23±14.06)(n＝3，P＝0.0229)。经葡萄籽提取物处理后，破骨标志分子 Itgb3、Acp5、Dcst、Ctsk mRNA水平均显著降低， TRAP阳性细胞数目减少， TRAP活性由(2.52±0.39)下降至(0.91±0.10)(n＝3，P＝0.0165)，TRAP活性显著降低；与对照组细胞相比， GSE组细胞分泌的 c?TX量由(1.01±0.11)nmol/L下降至(0.49±0.06)nmol/L(n＝3，P＝0.0028)，c?TX分泌显著减少。结论葡萄籽提取物有效抑制破骨细胞分化、形成及活性，葡萄籽提取物是潜在的预防治疗骨质疏松的营养补充剂。 |
| 英文摘要: |
| Objective To detect the effects of grape seed extract(GSE)on differentiation，formation and function of osteoclasts. Methods The mRNA levels of osteoclastic markers were examined by realtime qPCR.The formation of osteoclasts was determinedby tartrate?resistant acid phosphatase(TRAP)staining and TRAP activity assays.The function of osteoclasts was assessed by quan?tification of c?TX secreted into the culture medium.Results After GSE treatment，in comparison with the control group，the mRNA levels of osteoclastic markers were significantly down?regulated［Itgb3 mRNA from(216.59±34.61)to(104.55±9.54)，n＝3，P＝0.035 5，Acp5 from(103.37±14.77)to(78.96±13.15)n＝3，P＝0.0351，Dcst fro(304.85±14.77)to(65.03±11.75)n＝3，P＝0.0003，Ctsk from(290.23±41.65)to(132.23±14.06)n＝3，P＝0.0229］，the TRAP positive cells were decreased，TRAP activity was impaired［from(2.52±0.39)to(0.91±0.10)n＝3，P＝0.0165］，and c?TX secretion was inhibited［from(1.01±0.11)nmol/L to(0.49±0.06)nmol/L，n＝3，P＝0.002 8］in RAW264.7 cells of GSE group.Conclusion Grape seed extract inhibits the differen? tiation，formation and activity of osteoclasts，which is a potential supplement for the prevention and treatment of osteoporosis. |
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