| 邓颖,黎金凤,陈志雄,等.胰高血糖素样肽1对2型糖尿病大鼠骨髓间充质干细胞成骨和成脂分化的影响[J].安徽医药,2020,24(9):1708-1711. |
| 胰高血糖素样肽1对2型糖尿病大鼠骨髓间充质干细胞成骨和成脂分化的影响 |
| Effect of glucagon-like peptide-1on osteogenesis and adipogenesis differentiation of bone marrow mesenchymal stem cells from rats in type 2 diabetes mellitus |
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| DOI：10.3969/j.issn.1009-6469.2020.09.004 |
| 中文关键词: 糖尿病， 2型 间质干细胞 胰高血糖素样肽 -1 成骨分化 成脂分化 大鼠， Sprague-Dawley |
| 英文关键词: Diabetes mellitus,type 2 Mesenchymal stem cells Glucagon-like peptide-1 Osteogenesisdifferentiation Adipogenesisdifferentiation Rats,Sprague-Dawley |
| 基金项目:江西省卫生计生委科技计划项目( 20165024) |
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| 中文摘要: |
| 目的探讨胰高血糖素样肽 -1(GLP-1)对 2型糖尿病( T2DM)大鼠骨髓间充质干细胞( BMSCs)成骨和成脂分化的作用。方法将 20只 SD大鼠按随机数字表法分为正常组及 T2DM组，每组 10只，高脂饲料喂养 1个月联合链脲佐菌素( STZ)溶液注射制备 T2DM模型大鼠，原代分离培养正常大鼠和 T2DM大鼠 BMSCs，并采用流式细胞术鉴定细胞。随后将正常大鼠 BMSCs分为正常大鼠 BMSCs(正常组)正常大鼠 BMSCs+10 nmol/L GLP-1(正常组 +10 nmol/L GLP-1)正常大鼠 BMSCs+30 nmol/L GLP-1(正常组+30nmol/LGLP-1)，。T2DM模型大鼠 BMSCs分为 T2DM模型大鼠 BMSCs(模型组)，，T2DM模型大鼠 BMSCs+ 10nmol/L GLP-1(模型组 +10 nmol/L GLP-1)，T2DM模型大鼠 BMSCs+30 nmol/L GLP-1(模型组 +30 nmol/L GLP-1)。检测各组细胞的碱性磷酸酶( ALP)活性及成脂、成骨分化情况。结果分离获得的大鼠 BMSCs细胞呈纺锤形或长梭形，核内可见 1~2个核仁。流式细胞术结果表明正常组大鼠及模型组大鼠的 BMSCs均一性较好，纯度高。与正常组( 2.81±0.04)比较，正常组 +10 nmol GLP-1(4.92±0.07)及正常组 +30 nmol GLP-1(6.76±0.02)的 ALP活性升高，模型组( 3.54±0.09)ALP活性降低；与模型组比较，模型组 +10 nmol GLP-1(5.11±0.10)及模型组 +30 nmol GLP-1(5.80±0.17)的 ALP活性升高( F＝23.56，P＝0.000)。与正常组(0.46±0.05)比较，正常组 +10 nmol GLP-1(0.53±0.05)及正常组 +30 nmol GLP-1(0.64±0.02)的 OD值升高，模型组( 0.09±0.01) OD值降低；与模型组比较，模型组 +10 nmol GLP-1(0.16±0.03)及模型组 +30 nmol GLP-1(0.33±0.03)的 OD值升高( F＝18.39， P＝0.001)。与正常组( 0.29±0.05)比较，正常组 +10 nmol GLP-1(0.16±0.05)及正常组 +30 nmol GLP-1(0.09±0.02)的 OD值降低，模型组( 0.66±0.01)OD值升高；与模型组比较，模型组 +10 nmol GLP-1(0.43±0.03)及模型组 +30 nmol GLP-1(0.34±0.03)的 OD值降低( F＝29.41，P＝0.000)。结论 GLP-1对 T2DM大鼠 BMSCs成骨分化具有促进作用，对成脂分化具有抑制作用。 |
| 英文摘要: |
| Objective To explore effect of glucagon-like peptide-1(GLP-1)on osteogenesis and adipogenesis differentiation of bone marrow mesenchymal stem cells(BMSCs)inrats with type 2 diabetes mellitus(T2DM).Methods Twenty SD rats were randomly divided into two groups:normal group and T2DM group according to the random number table method.T2DM rats was establishedby high-fat diet feeding for 1 month combined with streptozotocin(STZ)injection.BMSCs of normal rats and T2DM rats were isolat- ed and cultured in primary generation，and BMSCs were identified by flow cytometry.Normal rats BMSCs were divided into normal rats BMSCs(normal group)，normal rats BMSCs+10 nmol/L GLP-1(normal group+10 nmol/L GLP-1)，normal rats BMSCs+30 nmol/L GLP-1(normal group+30 nmol/L GLP-1).BMSCs of T2DM rats were divided into BMSCs of T2DM rats(model group)BMSCs+10 nmol/L GLP-1(model group + 10 nmol/L GLP-1)and BMSCs + 30 nmol/L GLP-1(model group + 30 nmol/L GLP-1)，in T2DM model rats.After glucagon-like peptide-1(GLP-1)was added to BMSCs of normal rats and model rats，Alkaline phospha- tase(ALP)activity，differentiation of BMSCs after induction in each group was detected.Results The isolated BMSCs cells were spindle-shaped or spindle-shaped with 1-2 nucleoli in the nucleus.The results of flow cytometry showed the BMSCs of normal groupand model group had good homogeneity and high purity.Compared with normal group(2.81±0.04)， the activity of ALP was in- creased in normal group+10 nmol/L GLP-1(4.92±0.07)and normal group+30 nmol/L GLP-1(6.76±0.02)，the activity of ALP was decreased in model group(3.54±0.09).Compared with modelgroup，the activity of ALP was increased in normal group+10 nmol/L GLP-1(5.11±0.10)and normal group+30 nmol/L GLP-1(5.80±0.17)(F＝23.56，P＝0.000).Compared with normal group(0.46±0.05)，OD value was increased in normal group+10 nmol/L GLP-1(0.53±0.05)and normal group+30 nmol/L GLP-1(0.64±0.02)， OD value was decreased in model group(0.09±0.01).Compared with modelgroup，OD value was increased in normal group+10 nmol/L GLP-1(0.16±0.03)and normal group+30 nmol/L GLP-1(0.33±0.03)(F＝18.39，P＝0.001).Compared with normal group(0.29±0.05)，OD value was decreased in normal group+10 nmol/L GLP-1(0.16±0.05)and normal group+30 nmol/L GLP-1(0.09±0.02)，OD value was increased in model group(0.66±0.01).Compared with modelgroup，OD value was decreased in normal group+10 nmol/L GLP-1(0.43±0.03)and normal group+30 nmol/L GLP-1(0.34±0.03)(F＝29.41，P＝0.000).Conclusion GLP-1 could promote the osteogenic differentiation of BMSCs and inhibit the adipogenic differentiation of BMSCs in rats with T2DM. |
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