| 文杨,张军,王毅.麻杏石甘汤对重症肺炎大鼠肺组织炎症的影响及其机制[J].安徽医药,2020,24(9):1725-1728. |
| 麻杏石甘汤对重症肺炎大鼠肺组织炎症的影响及其机制 |
| Effect of Maxing Shigan decoction on inflammation in lung tissue of rats with severe pneumonia and its mechanism |
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| DOI：10.3969/j.issn.1009-6469.2020.09.007 |
| 中文关键词: 麻杏石甘汤 肺炎 克雷伯菌感染 炎症 NF-κB信号通路 大鼠 |
| 英文关键词: Maxing Shigan decoction Pneumonia Klebsiella infections Inflammation NF-κB signaling pathway Rats |
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| 中文摘要: |
| 目的探讨麻杏石甘汤( MGD)对重症肺炎大鼠炎症的影响及其机制。方法按照随机数字表法将正常饲养的 80只大鼠分成对照组、模型组、模型 +0.9%氯化钠溶液组、模型 +MGD组，每组 20只。对照组:正常饲养的 20只大鼠；模型组:用 1 mL注射器穿刺气管缓慢滴入 0.3 mL新鲜配制的肺炎克雷伯菌混悬液将剩余 60只大鼠进行重症肺炎模型制备；模型 +0.9%氯化钠溶液组:将 20只模型组大鼠进行等量的 0.9%氯化钠溶液灌胃；模型 +MGD组:将 20只模型组大鼠进行 MGD灌胃。用血气分析检测分析血气指标；酶联免疫吸附试验( ELISA)检测大鼠血清中肿瘤坏死因子 α(TNF-α)、白细胞介素 6(IL-6)、白细胞介素 1β(IL-1β)的含量；蛋白质印迹法检测肺组织中核因子 κB抑制蛋白( IκBα)、 p65、磷酸化 IκBα(p-IκBα)的蛋白表达。结果成功构建肺炎克雷伯菌诱导的重症肺炎大鼠；与模型 +0.9%氯化钠溶液组大鼠相比，模型 +MGD组大鼠动脉血氧分压( PaO2)［(11.89±0.83)kPa比( 8.89±0.62)kPa，P＜0.05］、血氧饱和度( SaO2)［(95.95±0.86)%比( 83.05±8.62)%，P＜0.05］显著升高，动脉血二氧化碳分压( PaCO2)显著降低［(4.02±0.37)kPa比(6.49±0.59)kPa，P＜0.05］肺湿干比［(4.29±0.40)%比( 5.28±0.48%)，P＜0.05］、 TNF-α［(103.24±8.42)ng/mL比( 448.06±41.12)ng/mL，P＜0.05］、 IL-6［7±8.11)pg/mL比( 209.51±18.18pg/mL)P＜(85.8，0.05］、 IL-1β［( 218.54±19.33)ng/mL比( 528.31±49.11)ng/mL，P＜0.05］的含量均显著降低；重要的是， IκBα的表达明显上调，［(1.86±0.11)比( 0.25±0.02)P＜0.05］p65［(0.29±0.02)比( 4.10±0.04)P＜0.05］、 p-IκBα［(0.31±0.03)比( 2.94±0.21)，P＜0.05］的表达明显下调。结论 MGD，可抑制重，症肺炎大鼠的炎症分泌，其机制，可能与抑制 NF-κB信号通路相关。 |
| 英文摘要: |
| Objective To investigate the effect and mechanism of Maxing Shigan decoction(MGD) on inflammation of severe pneumonia in rats.Methods According to the random number table method，80 rats fed normally were divided into control group， model group，model+0.9% sodium chloride solution group，and model + MGD group，with 20 rats in each group.Control group:20 rats normally reared；Model group:Use a 1 mL syringe to puncture the trachea and slowly drop 0.3 mL of freshly prepared Klebsiel-la pneumoniae suspension into the remaining 60 rats for severe pneumonia model preparation；Model+0.9% sodium chloride solu- tion group:20 rats in the model group were given the same amount of 0.9% sodium chloride solution by gavage；model+MGD group: 20 rats in the model group were given MGD by gavage.The blood gas index was analyzed.The contents of tumor necrosis factor-α(TNF-α)，interleukin 6(IL-6)and interleukin 1β(IL-1β)in serum were detected by enzyme-linked immunosorbent assay(ELI- SA).The protein expressions of nuclear factor kappa B inhibitor(IκBα)，p65，phosphorylated IκBα(p-IκBα)in lung tissue were detected by Western blot.Results The rats with severe pneumonia induced by Klebsiella pneumoniae were successfully construct-ed.Compared with the model+0.9% sodium chloride solution group，the arterial partial pressure of oxygen(PaO2)［( 11.89±0.83) kPa vs.(8.89±0.62)kPa，P＜0.05］，blood oxygen saturation(SaO2)［(95.95±0.86)% vs.(83.05±8.62)%，P＜0.05］in the model+ MGD group increased；arterial partial pressure of carbon dioxide(PaCO2)significantly decreased［( 4.02±0.37)kPa vs.(6.49±0.59)kPa，P＜0.05］； lung wet-to-dry ratio［( 4.29±0.40)% vs.(5.28±0.48)%，P＜0.05］TNF-α［( 103.24±8.42)ng/mL vs.(448.06±41.12)ng/mL，P＜0.05］，IL-6［(85.87±8.11)pg/mL vs.(209.51±18.18)pg/mL，P＜005］，IL-1β［(218.54±19.33)ng/mL vs.(528.31±49.11)ng/mL，P＜0.05］reduced；importantly，the expression of IκBα was up-regulated［(1.86±0.11)vs.(0.25±0.02)， P＜0.05］，while p65［(0.29±0.02)vs.(4.10±0.04)P＜0.05］and p-IκBα［(0.31±0.03)vs.(2.94±0.21)，P＜0.05］was down-reg-ulated.Conclusion MaxingShigandecoctioncaninh，ibit the inflammatory secretion of severe pneumonia rats，and its mechanism may be related to the inhibition of NF-κB signaling pathway. |
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