| 王晓娟,袁君,黄庆,等.反相高效液相色谱法测定人血清中拉莫三嗪的浓度[J].安徽医药,2020,24(9):1732-1734. |
| 反相高效液相色谱法测定人血清中拉莫三嗪的浓度 |
| Determination of lamotrigine in human serum by RP-HPLC |
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| DOI：10.3969/j.issn.1009-6469.2020.09.009 |
| 中文关键词: 拉莫三嗪/分析 色谱法，高压液相 血药浓度 |
| 英文关键词: Lamotrigine/analysis Chromatography,high pressure liquid Serum concentration |
| 基金项目:淮南市科技计划项目( 2013A4307) |
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| 中文摘要: |
| 目的建立人血清拉莫三嗪浓度的反相高效液相色谱方法。方法以艾司唑仑为内标，乙酸乙酯为萃取剂， C18柱分析柱，流动相甲醇∶磷酸二氢钠缓冲液( 56∶44，pH 6.0)流速 1.0 mL/min；检测波长 210 nm，柱温 30 ℃。结果拉莫三嗪在 0.10～100.00 mg/L范围浓度内线性关系良好( r＝0.999 6)，量浓度下限为 0.10 mg/L，高、中、低质控样品相对回收率在 80%～120%定，之间，提取回收率分别为 90.95%、90.98%、95.10%，日内、日间相对标准偏差( RSD)均＜ 5%。结论该方法准确，分析时间较短，线性范围大，可用于服用拉莫三嗪病人的血清浓度的临床常规监测。 |
| 英文摘要: |
| Objective To establish an Reversed-phase High Performance Liquid Chromatography(RP-HPLC)method for the con- centration of lamotrigine in human serum.Methods Estazolam was used as internal standard，ethyl acetate was used as extractant， and lamotrigine was analyzed via a C18column and mobile phase methanol∶sodium hydrogen phosphate buffer(56∶44，pH 6.0)un- der the flow rate of 1.0mL/min，at the column temperature of 30℃.The detection occurred at wave length of 210nm.Results La- motrigine has a good linear relationship within the concentration range of 0.10-100.00 mg/L(r＝0.999 6)with a quantitative lower limit of 0.10 mg/L.The extraction recoveries rate of high，medium and low quality control samples was in the range of 80%-120%. The extraction recovery rates were 90.95%，90.98% and 95.10% respectively，，and the relative standard deviations(RSD)within and between days were＜5%.The intraday and intraday precisions were less than 5%.Conclusion The method is accurate and sensitive，the analysis time is short，and the linear range is large.It can be used to detect the serum concentration of lamotrigine for its clinical applications. |
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