| 张捷,龚庆豪,章波,等.多功能蛋白聚糖在胃癌中的表达及其临床预后[J].安徽医药,2020,24(9):1798-1803. |
| 多功能蛋白聚糖在胃癌中的表达及其临床预后 |
| Expression of versican and its clinical prognosis in gastric cancer |
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| DOI：10.3969/j.issn.1009-6469.2020.09.026 |
| 中文关键词: 胃肿瘤/病因学 多功能蛋白聚糖类 癌症基因组图谱 预后 |
| 英文关键词: Stomach neoplasms/etiology Versicans The cancer genome atlas Prognosis |
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| 中文摘要: |
| 目的探索多功能蛋白聚糖( Versican，VCAN)在胃癌中的表达及临床预后意义。方法下载整理癌症基因组图谱( TC- GA)的胃癌相关数据，联合 Oncomine数据库分析胃癌组织中 VCAN mRNA的表达水平，探究 VCAN mRNA表达与胃癌临床病理参数关系，采用 Kaplan-Meier和 Cox回归模型分析胃癌病人预后的影响因素。采用基因集富集分析( GSEA)法分析 VCAN调控的相关信号通路。结果 VCAN mRNA在胃癌组织中的表达水平( 15.138±0.654)明显高于正常胃组织( 3.678±0.346)差异有统计学意义( t＝5.103，P＜0.001)。 VCAN mRNA表达与 TNM分期( F＝3.192，P＝0.038)、 T分期( F＝4.703，P＝0.003)及病，理分级( t＝2.642，P＝0.009)相关。 VCAN mRNA高表达病人与低表达病人相比生存率较低，差异有统计学意义( P＝0.015)Cox多因素分析结果表明 VCAN表达和年龄是病人不良预后的独立风险因素(均 P＜0.05)。 GSEA分析发现在 VCAN高表达的，主要富集在丝裂原活化蛋白激酶( MAPK)，Janus激酶 /信号转导和转录激活因子( JAK-STAT)，Wnt，Toll样受体和哺乳动物雷帕霉素靶蛋白( mTOR)等信号通路中。结论 VCAN在胃癌组织中高表达，其高表达与胃癌病人的不良预后密切相关， VCAN可能在胃癌的发生发展中发挥重要作用。 |
| 英文摘要: |
| Objective To study the expression of versican(VCAN)levels in gastric cancer and its correlation with the clinical prognosis of gastric cancer.Methods The expression data of VCAN and its clinical information in gastric cancer were downloadedfrom TCGA dataset.Public data bases including Oncomine and the cancer genome atlas(TCGA) were used to assess VCAN mRNA expression in gastric cancer.Then the relationship between the expression of VCAN and clinicopathological characteristicsand the prognostic value of VCAN was analyzed.Then the relationship between the expression of VCAN and clinicopathologicalcharacteristics.The prognostic value of VCAN was analyzed using Kaplan-Meier method and Cox regression model.Gene set enrich- ment analysis(GSEA)was used to predict the regulatory gene sets and the related signal pathways regulated by VCAN.Results The expression of VCAN mRNA in gastric cancer tissues(15.138±0.654)was higher than that in non-cancerous tissues(3.678± 0.346)( t＝5.103，P＜0.001).High expression of VCAN was significantly correlated with TNM stage(F＝3.192，P＝0.038)， T stage(F＝4.703，P＝0.003)and histology grade(t＝2.642，P＝0.009).A higher expression of VCAN demonstrated significantlypoorer overall survival in gastric cancer patients than in those with lower VCAN expression(P＝0.015).Cox multivariate analyses suggested that VCAN expression and age were independent predictors for overall survival(both P＜0.05).VCAN could regulate the gene sets such as MAPK signaling pathway，JAK-STAT signaling pathway，Wnt signaling pathway，toll like receptor signaling path- way and mTOR signaling pathway.Conclusions The expression of VCAN in gastric cancer was up-regulated.As an independent risk factor，high expression of VCAN indicates a poor prognosis for patients with gastric cancer.VCAN may play an important rolein the occurrence and development of gastric cancer. |
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