| 魏莹,肖莉,杨兰,等.不同产地佛手的香叶木苷及橙皮苷含量同时测定法[J].安徽医药,2021,25(1):32-34. |
| 不同产地佛手的香叶木苷及橙皮苷含量同时测定法 |
| Simultaneous determination of diosmin and hesperidin in bergamot from different areas |
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| DOI：10.3969/j.issn.1009?6469.2021.01.008. |
| 中文关键词: 色谱法，高压液相 芸香科 含量测定 佛手 香叶木苷 橙皮苷 |
| 英文关键词: Chromatography,high pressure liquid Rutaceae Content determination Bergamot Diosmin Hesperidin |
| 基金项目:南充市校科技战略合作专项(19SXHZ0231)；川北医学院2015年校级科研发展项目(CBY15?A?YB22) |
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| 中文摘要: |
| 目的佛手药材中香叶木苷和橙皮苷含量测定方法的建立。方法 2015年 9月至 2018年 6月，以 DiamonsilC18(250 mm×4.6 mm，5 μm)为色谱柱，乙腈(A)-0.1%醋酸水(B)(20∶80)等度洗脱为流动相， 285 nm为检测波长， 1.0 mL/min的流速， 25 ℃的柱温， 10 μL的进样量。结果香叶木苷、橙皮苷进样量分别在 12.32~616 ng(r＝0.999 8)8.128~1 016 ng(r＝0.999 9)范围内与峰面积呈良好线性关系，平均回收率(n＝6)分别为 99.32%、100.87%；相对标准偏差(RSD)，分别为 2.29%、2.24%。结论该法简便、准确、可靠，可作为佛手药材中香叶木苷、橙皮苷含量测定的有效方法。 |
| 英文摘要: |
| Objective Establishment of a method for determination of diosmin and hesperidin in bergamot medicinal materials. Methods From September 2015 to June 2018，the chromatographic column was Diamonsil C18(250 mm×4.6 mm，5 μm)，mobile phase was acetonitrile(A)-0.1% acetic acid water(B)inisometric elution(20:80).The detection wavelength was 285 nm and the flow rate wass 1.0 mL min?1at 25 ℃，and the injection volume was 10 μL.Results The contents of diosmin and hesperidin had good linear relationship with the peak area in the ranges of 12.32~616 ng(r＝0.999 8)and 8.128~1 016 ng(r＝0.999 9)respective? ly，and the average recoverie rate(n＝6)of five components was 99.32% with RSD of 2.29%and 100.87% with RSD of 2.24% re? spectively.Conclusion The method is simple，accurate and reliable，and can be used as an effective method for the determination of diosmin and hesperidin in bergamot. |
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