| 李孝平,李伟,高芳芳,等.山楂多糖提取物上调 miR-146a-5p抑制 Wnt/β-catenin信号通路影响胃癌细胞增殖和凋亡[J].安徽医药,2021,25(2):326-330. |
| 山楂多糖提取物上调 miR-146a-5p抑制 Wnt/β-catenin信号通路影响胃癌细胞增殖和凋亡 |
| Up-regulation of miR-146a-5p by Hawthorn polysaccharide extract inhibits Wnt/β-catenin signaling pathway to affect proliferation and apoptosis of gastric cancer cells |
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| DOI：10.3969/j.issn.1009-6469.2021.02.028. |
| 中文关键词: 山楂属 植物提取物 miR-146a-5p Wnt/β-catenin信号通路 胃癌 增殖 凋亡 |
| 英文关键词: Crataegus Plant extracts miR-146a-5p Wnt/β -catenin signaling pathway Gastric cancer Proliferation Apoptosis |
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| 中文摘要: |
| 目的探讨山楂多糖提取物对胃癌细胞增殖和凋亡的影响及机制。方法用浓度分别为 200、400、800 μg/mL的山楂多糖提取物处理 AGS细胞，作为不同浓度山楂多糖提取物组；正常培养的细胞作为对照(NC)组；用 800 μg/mL的甘露醇处理作等渗对照，记为 800 μg/mL甘露醇组。将 miR-NC、miR-146a-5p转染至 AGS细胞中记为 miR-NC组、 miR-146a-5p组；将 anti-miRNC、anti-miR-146a-5p转染至 AGS细胞中再用浓度为 800 μg/mL的山楂多糖提取物处理，记为 anti-miR-NC+山楂多糖提取物组、 anti-miR-146a-5p+山楂多糖提取物组。四甲基偶氮唑盐比色法( MTT)检测细胞增殖率；流式细胞术检测细胞凋亡；实时荧光定量 PCR(RT-qPCR)检测 miR-146a-5p表达水平。结果与对照组( 100.10±10.02)%相比， 200、400、800 μg/mL山楂多糖提取物组胃癌细胞 AGS的增殖率( 85.12±8.51)%、(68.22±6.82)%和( 42.11±4.21)%降低( F = 31.314，P < 0.05)。不同浓度山楂多糖提取物处理的胃癌细胞 AGS中细胞增殖率降低，细胞凋亡率升高， miR-146a-5p表达水平升高( P < 0.05)。高表达 miR-146a5p，细胞增殖率降低，细胞凋亡率升高( P < 0.05)。低表达 miR-146a-5p部分逆转了山楂多糖提取物对胃癌细胞 AGS增殖和凋亡的影响。山楂多糖提取物处理的胃癌细胞 AGS中 β-catenin表达水平降低，低表达 miR-146a-5p逆转了山楂多糖提取物对 βcatenin表达的抑制作用。结论山楂多糖提取物可抑制胃癌细胞 AGS增殖，促进细胞凋亡，其机制可能与 miR-146a-5p和 Wnt/β-catenin信号通路有关。 |
| 英文摘要: |
| Objective To investigate the effect and mechanism of polysaccharide extract of Cornus officinalis on proliferation andapoptosis of gastric cancer cells.Methods AGS cells were treated with hawthorn polysaccharide extracts at concentrations of 200,400, and 800 μg/mL, respectively, as different concentrations of hawthorn polysaccharide extract group; normal cultured cells wereused as control (NC) group, treated with 800 μg/mL mannitol as an isotonic control, recorded as 800 μg/mL mannitol group. miR-NC and miR-146a-5p were transfected into AGS cells and recorded as miR-NC group and miR-146a-5p group; anti-miR-NC and anti-miR146a-5p were transfected into AGS cells and treated with hawthorn polysaccharide extract at a concentration of 800 μg/mL, and recorded as an anti-miR-NC+hawthorn polysaccharide extract group and an anti-miR-146a-5p+ hawthorn polysaccharide extract group. Cellproliferation rate was detected by MTT assay; apoptosis was detected by flow cytometry; real-time quantitative PCR (RT-qPCR) was used to detect the expression of miR-146a-5p.Results The cell proliferation rate of gastric cancer cells AGS treated with different concentrations of hawthorn polysaccharide extract was decreased [(100.10±10.02)% vs. (85.12±8.51)%, (68.22±6.82)%, (42.11±4.21)%, P < 0.05], the apoptosis rate was increased, and the expression of miR-146a-5p was increased (P < 0.05). High expression of miR-146a5p, cell proliferation rate was decreased, apoptosis rate was increased (P < 0.05). Low expression of miR-146a-5p partially reversed theeffect of hawthorn polysaccharide extract on proliferation and apoptosis of gastric cancer cell AGS. The expression of β-catenin in gastric cancer cells AGS treated with polysaccharide extract of hawthorn was decreased, the low expression of miR-146a-5p reversed the inhibitory effect of hawthorn polysaccharide extract on β-catenin expression.Conclusions Hawthorn polysaccharide extract can inhibit the proliferation of gastric cancer cells AGS and promote cell apoptosis. The mechanism may be related to miR-146a-5p and Wnt/βcatenin signaling pathway. |
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